並列タイトル等胃癌におけるERCC1の主要なアイソフォームを検出する新たな抗ERCC1モノクローナル抗体の作製
授与機関名Nagasaki University (長崎大学)
一般注記Identifying patients resistant to cisplatin treatment is expected to improve cisplatin-based chemotherapy for various types of cancers. Excision repair cross-complementing group 1 (ERCC1) is involved in several repair processes of cisplatin-induced DNA crosslinks. ERCC1 overexpression is reported as a candidate prognostic factor and considered to cause cisplatin resistance in major solid cancers. However, anti-ERCC1 antibodies capable of evaluating expression levels of ERCC1 in clinical specimens were not fully optimized. A mouse monoclonal antibody against human ERCC1 was generated in this study. The developed antibody 9D11 specifically detected isoforms of 201, 202, 203 but not 204, which lacks the exon 3 coding region. To evaluate the diagnostic usefulness of this antibody, we have focused on gastric cancer because it is one of the major cancers in Japan. When ERCC1 expression was analyzed in seventeen kinds of human gastric cancer cell lines, all the cell lines were found to express either 201, 202, and/or 203 as major isoforms of ERCC1, but not 204 by Western blotting analysis. Immunohistochemical staining showed that ERCC1 protein was exclusively detected in nuclei of the cells and a moderate level of constant positivity was observed in nuclei of vascular endothelial cells. It showed a clear staining pattern in clinical specimens of gastric cancers. Antibody 9D11 may thus be useful for estimating expression levels of ERCC1 in clinical specimens.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1380号 学位授与年月日:令和3年12月1日
Author: Takayuki Oishi, Yuka Sasaki, Ying Tong, Lichao Chen, Takae Onodera, Satoru Iwasa, Emiko Udo, Bungo Furusato, Hiroaki Fujimori, Shoji Imamichi, Takuya Honda, Tadayoshi Bessho, Junya Fukuoka, Kazuto Ashizawa, Kazuyoshi Yanagihara, Kazuhiko Nakao, Yasuhide Yamada, Nobuyoshi Hiraoka, Mitsuko Masutani
Citation: Global Health & Medicine, 3(4), pp. 226-235; 2021
identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01)
一次資料へのリンクURLhttps://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27105&item_no=1&attribute_id=70&file_no=1 （fulltext）
著作権情報© 2021 National Center for Global Health and Medicine
関連情報http://hdl.handle.net/10069/00041141
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）