Bile extracellular vesicles from end‑stage liver disease patients show altered microRNA content

• 資料種別: 博士論文
• タイトル: Bile extracellular vesicles from end‑stage liver disease patients show altered microRNA content
• 著者・編者: 中鋪, 卓
• 著者標目: 中鋪, 卓
• 出版事項: Asian Pacific Association for the Study of the Liver (APASL), Springer Nature
• 出版年月日等: 2021-09-01
• 出版年（W3CDTF）: 2021-09-01
• 並列タイトル等: 末期肝不全患者において胆汁中細胞外小胞の内包microRNAは変化する
• タイトル（掲載誌）: Hepatology International
• 巻号年月日等（掲載誌）: 15 3
• 掲載巻: 15
• 掲載号: 3
• 掲載ページ: 821-830
• ISSN（掲載誌）: ISSN : 1936-0533; ISSN : 1936-0541
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2021-09-01
• 報告番号: 甲医歯薬第1362号
• 学位: 博士(医学)
• 本文の言語コード: eng
• 件名標目: Biomarker; Nanoparticle tracking analysis; Exosome; Liver failure; Liver transplantation; Hepatocellular; carcinoma; Ultracentrifugation; Next-generation sequencing; Alcoholic liver disease; MiRNA-17
• 対象利用者: 一般
• 一般注記: 出版タイプ： VoR; Background: Extracellular vesicles (EVs) have recently attracted attention as novel diagnostic biomarkers and therapeutic tools. Several reports have correlated blood EVs with liver diseases. However, blood EVs do not reflect the liver state as it contains other systemically circulating EVs. Therefore, we focused on bile EVs, which are secreted directly from the liver, for the identification of potential biomarkers of liver failure.Methods: Bile samples were collected from liver transplant recipients (n = 21) diagnosed with end-stage liver disease (ESLD) and donors (normal liver, NL; n = 18) during transplantation. Bile EVs were extracted using ultracentrifugation.Results: Nanoparticle tracking analysis showed that bile EV concentration was significantly higher in recipients than in donors. Among recipients, bile EV concentration was remarkably higher in those with hepatocellular carcinoma. Next-generation sequencing revealed 461 and 465 types of microRNAs (miRNAs) in donor and recipient bile EVs, respectively, with no significant difference in diversity between the groups. Among 43 high-expression miRNAs, the expression of 86.0% of the miRNAs was higher in the bile EVs of recipients than in those of donors. Quantitative PCR validation showed that the levels of miR-17, miR-92a, miR-25, miR-423, and miR-451a significantly increased in bile EVs of recipients. Levels of miR-17 were remarkably higher in recipients with alcoholic ESLD.Conclusions: Secretion of EVs into the bile and their miRNA content increase in the ESLD state. Additionally, miRNA levels in bile EVs are not correlated with those in serum EVs. Bile EVs could be promising novel biomarkers for liver diseases.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1362号 学位授与年月日:令和3年9月1日; Author: Suguru Nakashiki, Satoshi Miuma, Hiroyuki Mishima, Hiroshi Masumoto, Masaaki Hidaka, Akihiko Soyama, Yasuko Kanda, Masanori Fukushima, Masafumi Haraguchi, Ryu Sasaki, Hisamitsu Miyaaki, Tatsuki Ichikawa, Mitsuhisa Takatsuki, Susumu Eguchi, Koh-ichiro Yoshiura & Kazuhiko Nakao; Citation: Hepatology International; 15(3), pp. 821–830; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-09-01)
• 一次資料へのリンクURL: fulltext; https://nagasaki-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=27254&item_no=1&attribute_id=70&file_no=1
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: © Asian Pacific Association for the Study of the Liver 2021
• 関連情報: http://hdl.handle.net/10069/00040949
• 関連情報（DOI）: 10.1007/s12072-021-10196-5; https://doi.org/10.1007/s12072-021-10196-5
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 長崎大学 : 長崎大学学術研究成果リポジトリ