並列タイトル等ビニル化合物生産微生物の探索とバイオベースポリマー開発への利用に関する研究
一般注記type:Thesis
The aim of this study is development of screening method for microbes producing vinyl compounds from environmental samples and synthesis of new biobased polymers using the vinyl compounds as monomers. Thus, the following studies were carried out.In Chapter 1, structure, function, and biosynthesis of itaconic acid (IA) derivatives with the vinylidene group were summarized and their perspectives for industrial use were discussed. In Chapter 2, a selective labeling method based on Mizoroki-Heck reaction for vinyl compounds and a simple TLC?densitometric method for the quantification of the vinyl compounds were developed.In Chapter 3, the selective labeling reaction developed in Chapter 2 was confirmed by an iodine test and the screening of IA-producing fungi from soils was performed using the developed method. Using this method, 37 strains were selected as IA-producing fungi from 240 strains isolated from soils. In Chapter 4, a direct screening method based on Thiol-ene reaction for microbes producing vinyl compounds from soil samples was developed. Soil samples were spread on an agar medium supplemented with TG and VA-044. Screening of soil samples using the screening method led to isolation of IA-producing fungi from a soil. This method was named DISCOVER (direct screening method based on coupling reactions for vinyl compound producers).In Chapter 5, 37 soil microbes isolated in Chapter 3 were further characterized. One out of the 37 strains was identified as A. niger S17-5. Identification of the IA derivatives produced by S17-5 revealed that they were identical to 9-HHIA and 10-HHIA. The antibacterial, anti-inflammatory, and cytotoxic properties of two IA derivatives were evaluated.In Chapter 6, IA and 10-HHIA were copolymerized at different ratios through aqueous free radical polymerization with KPS as an initiator, resulting in poly(IA-co-10-HHIA)s with different monomer compositions. This is the first time that biobased polyvinyls were synthesized using naturally occurring IA derivatives.In chapter 7, general review was described.In conclusion, developed screening methods permit selective isolation of microbes producing vinyl compounds from environment. And the vinyl compounds produced by microbes enable the development of bio-based polymers.
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）