一般注記type:text
Candida glabrata is a closely related species of Saccharomyces cerevisiae. Although S. cerevisiae is a non-pathogenic species, C. glabrata is an opportunistic pathogenic species which increase mortality rates of hospital patients. One of the major features of C. glabrata is ifs biofilms formation and drug resistance. For example, this fungus has a high resistance to general azole fungicides used in the treatment of candidiasis. Considering the evolutionary gap between Candida and Saccharomyces species, it can be assumed that C. glabrata's ability to infect humans emerged independently from those of other Candida species. Then, we aimed to reveal the mechanism of stress resistance by comparing C. glabrata and S.cerevisiae. Firstly, we created a stoichiometric model of C. glabrata based on community model of the S. cerevisiae using BLASTP and R. As a result, we showed a part of the differences in the metabolism-related genes between S. cerevisiae and C. glabrata. The model will be compatible with Flux balance analysis tools based on flux balance analysis that is able to calculate the efficiency of metabolites and reaction pathway such as Flux-balance Analysis Based Simulations (FASIMU). Secondly, we compared the behavior of metabolites of C. glabrata and S. cerevisiae under osmotic stress conditions. As a result, we confirmed that C. glabrata has higher resistant to osmotic stress than S. cerevisiae. We hope that this study provide the insights about pathogenicity of C. glabrata and ifs drug resistance.
慶應義塾大学湘南藤沢キャンパス先端生命科学研究会 2014年度学生論文集
卒業論文ダイジェスト
identifier:SFC-RM2014-003
identifier:請求記号:
一次資料へのリンクURLhttps://koara.lib.keio.ac.jp/xoonips/modules/xoonips/download.php?koara_id=KO92001004-00000021-0166
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース(IRDB)(機関リポジトリ)