並列タイトル等Induction of tissue remodeling of reproductive organs using glialtem cell-derived factors
タイトル（掲載誌）平成23(2011)年度 科学研究費補助金 挑戦的萌芽研究 研究成果報告書 = 2011 Fiscal Year Final Research Report
一般注記金沢大学医薬保健研究域医学系
本研究では神経組織の再構築に関与するグリア幹細胞の生殖器官の再構築に対する促進効果の有無を検討した。その結果、h-TERT導入したヒト子宮内膜上皮細胞株の培養系で、グリア幹細胞の存在下にヒト子宮内膜上皮細胞間隙の接着および立体構造の再構築が促進されることが発見された。これらの知見はグリア幹細胞由来因子が生殖医療において新たな誘導因子として利用できる可能性を支持するものである。
Co-Investigator Nishio found that when stem cells of the neural epithelium-derived glia cells were transplanted into the severed site of the cordotomized mature rat, a rapid and successful regeneration of neuronal axons beyond the lesion site was achieved during several days. Central neural tissues construct tree-dimensional structures, creating complicated neural networks, which are composed of neuroepithelium-derived neurons and glial cells. In this respect, central nervous system has been far evolved than ordinary 2-dimensional epithelial layer and therefore it is speculated that the role of the glial cells in reconstruction will throw a new insight to the process of the reconstruction in reproductive organs. Based on this concept, this study was aimed to elucidate new mechanisms of developing and/or reconstructive processes of granulosa cells, endometrial epithelial cell and trophoblasts that belong to epithelial cell-lineages. First, we screened the effects of glial cell-derived factors on the culture of granulosa cells, endometrial epithelial cells, and trophoblasts. As a result, human telomerase reverse transcriptase(h-TERT)-transfected humam endometrial epithelial cell line(provided from Dr. Kyo at Kanazawa University) promoted its intercellular connection in the presence of glial stem cells derived from EGFP-transgenic rats, showing the possibility that immature glia cell-derived soluble factors can be one of the candidates to induce reconstruction of reproductive organs and to become a useful tool in the field of reproductive medicine.
研究課題/領域番号:23659778, 研究期間(年度):2011
出典：研究課題「グリア幹細胞由来因子を用いた生殖臓器の組織再構築誘導の試み」課題番号23659778（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23659778/23659778seika/）を加工して作成
一次資料へのリンクURLhttps://kanazawa-u.repo.nii.ac.jp/?action=repository_action_common_download&item_id=45233&item_no=1&attribute_id=26&file_no=1
関連情報https://kaken.nii.ac.jp/search/?qm=30252456
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659778/
https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23659778/23659778seika/
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）