並列タイトル等Elucidation of the mechanism of eosinophil activation in eosinophilic sinusitis
コウサンキュウセイ フクビコウエン ニオケル コウサンキュウ カッセイカ キジョ ノ カイメイ
一般注記2010-2011年度科学研究費助成事業(科学研究費補助金(若手研究(B)))研究成果報告書 課題番号：22791613 研究代表者：吉福孝介 (鹿児島大学医学部・歯学部附属病院講師)
好酸球浸潤の多い鼻茸群では好酸球浸潤の少ない鼻茸群と比較して、LPS刺激による線維芽細胞のVEGF 産生は有意に上昇していた。デキサメタゾン（10^{-6}mol）によりVEGF産生は有意に抑制された。VEGFの免疫染色において、線維芽細胞は好酸球浸潤の多い鼻茸群では有意に強く染まっていた。好酸球浸潤の多い鼻茸群では好酸球浸潤の少ない鼻茸群と比較してVEGF産生をする線維芽細胞数が多く、またLPS刺激などの外因因子が作用することでVEGF産生がより高い結果であった。VEGF産生が高いことは鼻茸の発生の一因である可能性があると思われた。
The mechanism by which eosinophils are selectively recruited in nasal polyp remains to be clarified.The exact mechanisms by which glucocorticoids exert their beneficial effect on nasal polyp remain to be clearly defined In the present study,the secreted levels of VEGF from the culured fibroblasts and VEGF in the nasal secretion were determined. The level were compared between Enp and NEnp and the role of those chemokines in the pathogenesis of nasal polyp is discussed. Tissues from nasal polyps obtained by biopsy was fixed in formarin and stained with hematoxylin and eosin, and the number of eosinophils was counted at ×400 magnification under light-microscopy. Three fields were examined for each section and the average was considered the number of eosinophils infiltrating the sample. Clinical features of Enp are complication with Asthma, bilateral nasal polyps and Ethmoidal sinus disease are often recognized in CT. These three items were examined, statistically significant difference in 40-60. So we classified samples having more than 60 eosinophils as Enp, having less than 40 eosinophils as Nenp in this study. 17 CRS with nasal polyp cases were included in the Nasal leakage study and 11 CRS with nasal polyp cases were included in the cultured fibroblast study. None of the subjects had taken oral steroids for more than year before the biopsy. Fibroblasts isolated from nasal polyps were cultured. After dexamethasone(DEX) pretreatment,stimulated with 10ng/ml of tumor necrosis factor-α(TNF-α)and interleukin-4(IL-4)for 24 hours or stimulated with 10μg/ml of LPS. After stimulation, culture supernatants were collected and concentration of VEGF were quantified by Enzyme linked immunosorbent assay(ELISA). Nasal secretions from nasal cavity were collected by JUHN TYM-TUP(Medtronic Xomed,Inc,Jacksonville,FL). Collected nasal secretions were diluted with 2 ml phosphate-buffered saline(PBS) and centrifuged at 350 ×g for 10 minutes. The supernatant was then harvested. VEGF levels in the supernatants were measured by ELISA. VEGF were significantly lower in the DEX10^{-6}DEX treated group compared to non-DEXtreated group. Conclusions: VEGF plays an important role in the selective recruitment of eosinophils in Enp. Dex may inhibit nasal polyp growth local reguration of decreased VEGF.
一次資料へのリンクURLhttps://ir.kagoshima-u.ac.jp/?action=repository_action_common_download&item_id=8150&item_no=1&attribute_id=16&file_no=1
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）