並列タイトル等Intranasal Immunization with Phosphorylcholine Suppresses Allergic Rhinitis in Mice
学位博士(医学)
Doctor of Philosophy in Medical Science
一般注記博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨
Objectives/Hypothesis: Intranasal immunization with phosphorylcholine (PC) is known to reduce IgE production. However, its effects on the occurrence of allergic rhinitis (AR) are unknown.
Objectives: This study was performed to evaluate the effects of PC-keyhole limpet hemocyanin (PC-KLH) and to examine the effects on the occurrence of AR in a murine model of AR.
Study Design: In vivo study using an animal model.
Methods: Forty-five female BALB/c mice were divided into 3 groups; those pretreated with intranasal administration of PC-keyhole limpet hemocyanin (PC-KLH) followed by intra-peritoneal sensitization and nasal challenge with ovalbumin (OVA) (Group A), those untreated with PC-KLH followed by sensitization and nasal challenge with OVA (Group B), and those untreated with PC-KLH or OVA as a control (Group C). Nasal symptoms, allergic inflammation in the nasal mucosa, OVA specific IgE production, and cytokine profile were compared among those three groups. Further, dendritic cells (DCs) were isolated from splenic cells and PC-KLH-stimulated IL-12p40 production was measured.
Results: The mice pretreated with PC-KLH showed lower allergic nasal symptoms and inflammation compared to untreated mice. The levels of total IgE and OVA-specific IgE in serum, and IL-4 production by nasal and splenic CD4+ T cells were significantly reduced by PC-KLH pretreatment. Furthermore, IL-12p40 production by DCs was induced by PC-KLH in a dose-dependent manner.
Conclusion: Intranasal administration of PC-KLH suppressed allergic inflammation in nasal mucosa and antigen-specific IgE production by down regulating Th2-type immune response. Intranasal immunization with PC might be useful to prevent AR and upper airway bacterial infection.
著作権情報© 2017 The American Laryngological, Rhinological and Otological Society, Inc.
連携機関・データベース国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）