RNAシークエンスによる食道扁平上皮癌・マイクロRNA発現プロファイルの作成 : 癌抑制型miR-143-5p と miR-143-3p が制御する癌促進型遺伝子の影響

• 資料種別: 博士論文
• タイトル: RNAシークエンスによる食道扁平上皮癌・マイクロRNA発現プロファイルの作成 : 癌抑制型miR-143-5p と miR-143-3p が制御する癌促進型遺伝子の影響
• 著者・編者: 和田, 真澄
• 著者標目: 和田, 真澄 ワダ, マスミ
• 出版事項: nature
• 並列タイトル等: RNA sequencing-based microRNA expression signature in esophageal squamous cell carcinoma : oncogenic targets by antitumor miR-143-5p and miR-143-3p regulation
• 授与機関名: 鹿児島大学
• 授与年月日: 2021-02-25
• 報告番号: 甲総研第584号
• 学位: 博士(医学); Doctor of Philosophy in Medical Science
• 本文の言語コード: eng
• 件名標目: microRNA; expression signature; esophageal squamous cell; carcinoma; miR-143-5p; miR-143-3p; high-mobility group AT-hook 2 (HMGA2); Keratin 80 (KRT80)
• 対象利用者: 一般
• 一般注記: 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Aberrantly expressed microRNAs (miRNAs) disrupt intracellular RNA networks and contribute to malignant transformation of cancer cells. Utilizing the latest RNA-sequencing technology, wenewly created the miRNA expression signature of esophageal squamous cell carcinoma (ESCC). A total of 47 miRNAs were downregulated in ESCC tissues, and these miRNAs were candidates for antitumor miRNAs in ESCC cells. Analysis of the signature revealed that several passenger strands of miRNAs were significantly downregulated in ESCC, e.g., miR-28-3p, miR-30a-3p, miR-30c-3p, miR-133a-3b, miR-139-3p, miR-143-5p, and miR-145-3p. Recent studies indicate that some passenger strands of miRNAs closely involved in cancer pathogenesis. In this study, we focused on both strands of pre-miR-143, and investigated their antitumor roles and target oncogenes in ESCC. Ectopic expression of miR-143-5p and miR-143-3p significantly attenuated malignant phenotypes (e.g., proliferation, migration, and invasive abilities) in ESCC cell lines. We revealed that 6 genes (HN1, HMGA2, NETO2, STMN1, TCF3 and MET) were putative targets of miR-143-5p regulation, and one gene (KRT80) was a putative target of miR-143-3p regulation in ESCC cells. Our ESCC miRNA signature and analysis strategy provided important insights into the molecular pathogenesis of ESCC. Masumi Wada, Yusuke Goto, Takako Tanaka, Reona Okada, Shogo Moriya, Tetsuya Idichi, Masahiro Noda, Ken Sasaki, Yoshiaki Kita, Hiroshi Kurahara, Kosei Maemura, Shoji Natsugoe & Naohiko Seki RNA sequencing-based microRNA expression signature in esophageal squamous cell carcinoma: oncogenic targets by antitumor miR-143-5p and miR-143-3p regulation Journal of Human Genetics volume 65, pages1019–1034 (2020) https://doi.org/10.1038/s10038-020-0795-x
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 鹿児島大学 : 鹿児島大学リポジトリ

目次

Result_Wada_Masumi_isk_584 other

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Abstract_和田_真澄__4517810419_2020 abstract