歯周組織の傷害はC57BL/6Jマウスにおいて三叉神経中脳路核の神経細胞死および三叉神経運動核の神経変性を生じる

• 資料種別: 博士論文
• タイトル: 歯周組織の傷害はC57BL/6Jマウスにおいて三叉神経中脳路核の神経細胞死および三叉神経運動核の神経変性を生じる
• 著者・編者: ダール, アシス
• 著者標目: ダール, アシス
• 出版事項: The Japan Society of Histochemistry and Cytochemistry
• 並列タイトル等: The periodontium damage induces neuronal cell death in the trigeminal mesencephalic nucleus and neurodegeneration in the trigeminal motor nucleus in C57BL/6Jmice
• 授与機関名: 鹿児島大学
• 授与年月日: 2021-03-25
• 報告番号: 甲総研第601号
• 学位: 博士(歯学)
• 本文の言語コード: eng
• 件名標目: trigeminal mesencephalic nucleus; trigeminal motor nucleus; degeneration; TDP-43; tooth extraction
• 対象利用者: 一般
• 一般注記: 出版タイプ： AM; 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; The trigeminal mesencephalic nucleus (Vmes) is involved in reflex proprioception of the periodontium and masticatory muscle function. We evaluated the effects of tooth loss on neurodegeneration of the Vmes and trigeminal motor nucleus (Vmo). Bilateral maxillary molars of 8-week-old C57BL/6J mice were extracted under anesthesia. Fluoro-Gold (FG) was retrogradely injected into the extraction sockets and masticatory muscle, and the Vmes was labeled with adeno-associated virus encoding green fluorescent protein (AAV-GFP) for anterograde labeling. After tooth extraction, sections were generated and immunohistochemically stained using antibodies against Piezo2, ATF3, cleaved caspase-3, and TDP-43. Neural projections of the Vmes to the periodontium were confirmed via FG and AAV-GFP labeling. At 1 month after tooth extraction, a significant decrease in the number of Piezo2-immunoreactive (IR) Vmes neurons was observed. ATF3-IR neurons were detected on day 5 after tooth extraction, and their number decreased on days 10 and 15. Dead cleaved caspase-3-IR neurons were found among Vmes nerve cells on days 7 and 12. In the Vmo, transient TDP-43 pathology appeared at 1 month after tooth extraction. These results indicate the existence of neural projections from the Vmes to the periodontium in mice, and that tooth loss induces the death of Vmes neurons followed by TDP-43 pathology in the Vmo. Therefore, tooth loss induces Vmes neuronal cell death, causing Vmo neurodegeneration and possibly affecting masticatory function. Ashis Dhar, Eriko Kuramoto, Makoto Fukushima, Haruki Iwai, Atsushi Yamanaka, Tetsuya Goto The Periodontium Damage Induces Neuronal Cell Death in the Trigeminal Mesencephalic Nucleus and Neurodegeneration in the Trigeminal Motor Nucleus in C57BL/6J Mice Acta Histochem. Cytochem. 54 (1): 11–19, 2021 https://doi.org/10.1267/ahc.20-00036 Copyright © 2021 The Japan Society of Histochemistry and Cytochemistry
• 記録形式（IMT）: application/pdf
• 一次資料へのリンクURL: https://ir.kagoshima-u.ac.jp/record/15846/files/Diss_アシス_ダール_ISK601_2020.pdf （fulltext）; https://ir.kagoshima-u.ac.jp/record/15846/files/Diss_%E3%82%A2%E3%82%B7%E3%82%B9_%E3%83%80%E3%83%BC%E3%83%AB_ISK601_2020.pdf
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 国立情報学研究所 : 学術機関リポジトリデータベース（IRDB）（機関リポジトリ）; https://irdb.nii.ac.jp
• 提供元機関・データベース: 鹿児島大学 : 鹿児島大学リポジトリ