A novel TK-NOG based humanized mouse model for the study of HBV and HCV infections.

• 資料種別: 博士論文
• タイトル: A novel TK-NOG based humanized mouse model for the study of HBV and HCV infections.
• 著者・編者: 小酒, 慶一
• 著者標目: 小酒, 慶一
• 出版年月日等: 2014-03-23
• 出版年（W3CDTF）: 2014-03-23
• 並列タイトル等: HBV及びHCV感染症研究のための新たなTK-NOGベースヒト化マウスモデルの検討
• 授与機関名: 広島大学
• 授与年月日: 2014-03-23
• 授与年月日（W3CDTF）: 2014-03-23
• 報告番号: 甲第6380号
• 学位: 博士(医学); Philosophy in Medical Science
• 博論授与番号: 15401甲第6380号
• 本文の言語コード: jpn
• 著者別名: Kosaka, Keiichi
• 件名標目: Human hepatocyte chimeric mouse; TK-NOG mouse; uPA-SCID mouse; Hepatitis B virus; Hepatitis C virus; Human serum albumin
• NDC: 490
• 対象利用者: 一般
• 一般注記: The immunodeficient mice transplanted with human hepatocytes are available for the study of the human hepatitis viruses. Recently, human hepatocytes were also successfully transplanted in herpes simplex virus type-1 thymidine kinase (TK)-NOG mice. In this study, we attempted to infect hepatitis virus in humanized TK-NOG mice and urokinase-type plasminogen activator-severe combined immunodeficiency (uPA-SCID) mice. TK-NOG mice were injected intraperitoneally with 6 mg/kg of ganciclovir (GCV), and transplanted with human hepatocytes. Humanized TK-NOG mice and uPA/SCID mice were injected with hepatitis B virus (HBV)- or hepatitis C virus (HCV)-positive human serum samples. Human hepatocyte repopulation index (RI) estimated from human serum albumin levels in TK-NOG mice correlated well with pre-transplantation serum ALT levels induced by ganciclovir treatment. All humanized TK-NOG and uPA-SCID mice injected with HBV infected serum developed viremia irrespective of lower replacement index. In contrast, establishment of HCV viremia was significantly more frequent in TK-NOG mice with low human hepatocyte RI (<70%) than uPA-SCID mice with similar RI. Frequency of mice spontaneously in early stage of viral infection experiment (8 weeks after injection) was similar in both TK-NOG mice and uPA-SCID mice. Effects of drug treatment with entecavir or interferon were similar in both mouse models. TK-NOG mice thus useful for study of hepatitis virus virology and evaluation of anti-viral drugs.; Keiichi Kosaka, Nobuhiko Hiraga, Michio Imamura, Satoshi Yoshimi, Eisuke Murakami, Takashi Nakahara, Yoji Honda, Atsushi Ono, Tomokazu Kawaoka, Masataka Tsuge, Hiromi Abe, C. Nelson Hayes, Daiki Miki, Hiroshi Aikata, Hidenori Ochi, Yuji Ishida, Chise Tateno, Katsutoshi Yoshizato, Tamito Sasaki and Kazuaki Chayama; A novel TK-NOG based humanized mouse model for the study of HBV and HCV infections; Biochemical and Biophysical Research Communications, Volume 441, Issue 1, 8 November 2013, Pages 230-235 (doi: 10.1016/j.bbrc.2013.10.040)
• 国立国会図書館永続的識別子: info:ndljp/pid/10164979; https://dl.ndl.go.jp/pid/10164979
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2016-08-02T06:56:17+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: http://ir.lib.hiroshima-u.ac.jp/00036221
• 掲載誌（URI）: http://ir.lib.hiroshima-u.ac.jp/00040621
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp