Mutation assay using single-molecule real-time (SMRT) sequencing technology

• 資料種別: 記事
• タイトル: Mutation assay using single-molecule real-time (SMRT) sequencing technology
• 著者・編者: Tomonari Matsuda; Shun Matsuda; Masami Yamada
• 出版事項: 日本 : Springer Nature
• 出版年月日等: 2015-09-01
• 出版年（W3CDTF）: 2015-09-01
• タイトル（掲載誌）: Genes and environment
• 巻号年月日等（掲載誌）: 37(15)
• 掲載巻: 37(15)
• ISSN（掲載誌）: 1880-7062
• ISSN-L（掲載誌）: 1880-7046
• 本文の言語コード: eng
• DOI: 10.1186/s41021-015-0017-5; https://doi.org/10.1186/s41021-015-0017-5
• 国立国会図書館永続的識別子: info:ndljp/pid/10224909; https://dl.ndl.go.jp/pid/10224909
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• 収集根拠: オンライン資料収集制度
• 受理日（W3CDTF）: 2016-12-02T08:00:31+09:00
• 保存日（W3CDTF）: 2016-11-21
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://genesenvironment.biomedcentral.com/articles?searchType=journalSearch&sort=PubDate&page=2
• 掲載誌（国立国会図書館永続的識別子）: info:ndljp/pid/10224894; https://dl.ndl.go.jp/pid/10224894
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• 要約等: Introduction: We present here a simple, phenotype-independent mutation assay using a PacBio RSII DNA sequencer employing single-molecule real-time (SMRT) sequencing technology. Salmonella typhimurium YG7108 was treated with the alkylating agent N-ethyl-N-nitrosourea (ENU) and grown though several generations to fix the induced mutations, the DNA was extracted and the mutations were analyzed by using the SMRT DNA sequencer. Results: The ENU-induced base-substitution frequency was 15.4 per Megabase pair, which is highly consistent with our previous results based on colony isolation and next-generation sequencing. The induced mutation spectrum (95% G:C→A:T, 5% A:T→G:C) is also consistent with the known ENU signature. The base-substitution frequency of the control was calculated to be less than 0.12 per Megabase pair. A current limitation of the approach is the high frequency of artifactual insertion and deletion mutations it detects. Conclusions: Ultra-low frequency base-substitution mutations can be detected directly by using the SMRT DNA sequencer, and this technology provides a phenotype-independent mutation assay.
• DOI: 10.1186/s41021-015-0017-5; https://doi.org/10.1186/s41021-015-0017-5
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: © 2015 Matsuda et al.; This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​4.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.
• 関連情報（URI）: http://hdl.handle.net/2433/210413; http://id.ndl.go.jp/digimeta/10224909; https://dl.ndl.go.jp/pid/10224909; http://link.springer.com/content/pdf/10.1186/s41021-015-0017-5.pdf; http://link.springer.com/article/10.1186/s41021-015-0017-5/fulltext.html; http://link.springer.com/content/pdf/10.1186/s41021-015-0017-5
• 参照: Revised methods for the Salmonella mutagenicity test; https://cir.nii.ac.jp/crid/1360574096464689152; A flexible and efficient template format for circular consensus sequencing and SNP detection; https://cir.nii.ac.jp/crid/1361699993949255936; Real-Time DNA Sequencing from Single Polymerase Molecules; https://cir.nii.ac.jp/crid/1361699995830719488; Detection of ultra-rare mutations by next-generation sequencing; https://cir.nii.ac.jp/crid/1362825893770114688; New tester strains of Salmonella typhimurium lacking O6-methylguanine DNA methyltransferases and highly sensitive to mutagenic alkylating agents; https://cir.nii.ac.jp/crid/1362825894536612096; DNA base changes and alkylation following in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea.; https://cir.nii.ac.jp/crid/1363388843650247296; A Benchmark Study on Error Assessment and Quality Control of CCS Reads Derived from the PacBio RS; https://cir.nii.ac.jp/crid/1363388843691516288; Detecting ultralow-frequency mutations by Duplex Sequencing; https://cir.nii.ac.jp/crid/1363388845570821376; Anticipated mutation assay using single-molecule real-time (SMRT) sequencing technology; https://cir.nii.ac.jp/crid/1390282680233168768; A Pilot Study for the Mutation Assay Using a High-throughput DNA Sequencer; https://cir.nii.ac.jp/crid/1390282680233647744
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: 学術機関リポジトリデータベース; https://irdb.nii.ac.jp; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles; 科学研究費助成事業データベース; https://kaken.nii.ac.jp/ja
• 書誌ID（NDLBibID）: 10224909; http://id.ndl.go.jp/bib/10224909
• NII論文ID: 120005749959