一般注記Recent research has shown that lactoferrin indirectly suppresses osteoclastogenesis by affecting osteoblasts and periodontal ligament fibroblasts. However, the mechanism by which lactoferrin directly affects osteoclastogenesis has not been reported. Therefore, the aim of this study was to examine the direct effects of lactoferrin on RANKL-induced osteoclast differentiation of murine osteoclastic RAW 264.7 cells. The number of osteoclasts was determined by counting the number of cells positive for TRAP staining. The effect of lactoferrin on the number of osteoclasts was measured, and the effect on the mRNA expression of osteoclast differentiation markers was assayed using real-time PCR. Lactoferrin decreased the number of osteoclasts (≥2 nuclei) and large osteoclasts (≥8 nuclei) in a dose-dependent manner without affecting the viability of RAW 264.7 cells. In addition, lactoferrin only mediated these effects within 48 h of the beginning of culturing the RAW 264.7 cells with RANKL. Lactoferrin also significantly inhibited RANKL-induced mRNA expression of osteoclasticdifferentiation genes such as NFATc1, RANK, DC-STAMP, and OC-STAMP.These findings suggest that lactoferrin directly suppresses osteoclastogenesis within 48 h of the beginning of culturing the RAW 264.7 cells with RANKL. The use of lactoferrin may be a novel and innovative therapy for treating bone diseases.
(主査) 教授 山崎 裕, 教授 鈴木 邦明, 教授 土門 卓文, 准教授 吉村 善隆
歯学研究科(口腔医学専攻)
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受理日(W3CDTF)2017-01-01T19:17:28+09:00
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