並列タイトル等Aberrant Expression of the Tight Junction Molecules Claudin-1 and Zonula Occludens-1 Mediates Cell Growth and Invasion in Oral Squamous Cell Carcinoma
口腔扁平上皮癌におけるタイト結合分子Claudin-1およびZonula Occludens-1の異常発現はがん細胞の増殖と浸潤を亢進させる
一般注記We reported that altered cell contact mediated by E-cadherin is an initial event in the pathogenesis of oral epithelial malignancies. To assess other effects of cell adhesion, we examined the expression levels of tight junction (TJ) molecules in oral carcinoma in-situ (CIS) and squamous cell carcinoma (SCC). To identify changes in the expression of TJ molecules, we conducted an analysis of the immunohistochemical profiles of claudin-1(CLDN-1) and zonula occludens-1 (ZO-1) in surgical specimens acquired from patients with oral SCC containing foci of epithelial dysplasia or from patients with CIS. We used\
immunofluorescence, western blotting, reverse transcriptase-polymerase chain reaction, and RNA interference (RNAi) to evaluate the functions of CLDN-1 and ZO-1 in cultured oral SCC cells. TJ molecules were not detected in normal oral epithelial tissues but were expressed in SCC/CIS cells. ZO-1 was localized within the nucleus of proliferating cells. When CLDN-1 expression was inhibited by transfecting cells with specific small interference RNAs,\
SCC cells dissociated, and their ability to proliferate and invade Matrigel was inhibited. In contrast, although RNAi-mediated inhibition of ZO-1 expression did not affect cell morphology, it inhibited cell proliferation and invasiveness. Our findings indicated that the detection of TJ molecules in the oral epithelia may serve as a marker for the malignant phenotype of cells in which CLDN-1 regulates proliferation and invasion.
学位の種類: 博士（歯学）. 報告番号: 甲第4222号. 学位記番号: 新大院博（歯）甲第364号. 学位授与年月日: 平成28年9月20日
新大院博（歯）甲第364号
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受理日（W3CDTF）2017-01-01T19:17:28+09:00
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