Ethanol attenuates vasorelaxation via inhibition of inducible nitric oxide synthase in rat artery exposed to interleukin-1β.

• 資料種別: 博士論文
• ISSN: 0960-3271
• タイトル: Ethanol attenuates vasorelaxation via inhibition of inducible nitric oxide synthase in rat artery exposed to interleukin-1β.
• 巻次・部編番号: Epub ahead of print
• 著者・編者: Yuui, Katsuya; Kudo, Risa; Kasuda, Shogo; Hatake, Katsuhiko
• 著者標目: Yuui, Katsuya; Kudo, Risa; Kasuda, Shogo; Hatake, Katsuhiko
• 出版事項: SAGE Publications
• 出版年月日等: 2015-10-22; Epub ahead of print
• 出版年（W3CDTF）: 2015-10-22
• 並列タイトル等: インターロイキン-1β暴露下のラットの血管におけるエタノールによる誘導型一酸化窒素合成酵素抑制を介した弛緩反応の抑制
• タイトル（掲載誌）: Human & experimental toxicology
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2016-03-15
• 授与年月日（W3CDTF）: 2016-03-15
• 報告番号: 甲第647号
• 学位: 博士（医学）
• 博論授与番号: 甲第647号
• 本文の言語コード: eng
• 件名標目: Ethanol; rat; superior mesenteric artery; interleukin-1β; relaxation response
• 対象利用者: 一般
• 一般注記: type:Thesis; Nitric oxide produced by inducible nitric oxide synthase (iNOS) regulates sepsis-induced hypotension. During septic shock, interleukin (IL)-1β is synthesized in endothelial cells and smooth muscle cells by endotoxin. Ethanol (EtOH) suppresses endotoxin-induced hypotension. The present study aimed to elucidate the effect of EtOH on gradual relaxation and iNOS expression induced by IL-1β in isolated rat superior mesenteric arteries (SMAs). Exposure to IL-1β–induced contraction in SMA rings, followed by a gradual relaxation of phenylephrine precontracted tone. Contraction was abolished by indomethacin (IM), cycloheximide (Chx), and endothelium denudation. In contrast, the gradual relaxation was abolished by NOS inhibitors, Chx, endothelium denudation, and inhibited by EtOH (50 and 100 mM). However, IM had no effect on relaxation. Western blot analysis demonstrated that iNOS expression was induced by IL-1β and was inhibited by EtOH and endothelium denudation. Furthermore, messenger RNA expression of iNOS, but not endothelial NOS, was inhibited by EtOH. These data suggest that IL-1β–induced contraction is mediated by thromboxane A2, whereas IL-1β–induced relaxation occurs via NO derived from iNOS. The endothelium plays an important role in vasorelaxation. Taken together, EtOH inhibits IL-1β–mediated vasorelaxation by suppressing endothelium iNOS expression. This study provides the first evidence of EtOH -induced inhibition of IL-1β–mediated vasorelaxation.; 博士（医学）・甲第647号・平成28年3月15日; © The Author(s); Copyright © 2016 by SAGE Publications; The definitive version is available at " http://dx.doi.org/10.1177/0960327115611944 "; identifier:Human & experimental toxicology [Epub ahead of print] pii: 0960327115611944(2015 Oct 22); identifier:09603271; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3184; identifier:Human & experimental toxicology, Epub ahead of print: pii: 0960327115611944
• DOI: info:doi/10.1177/0960327115611944; https://doi.org/info:doi/10.1177/0960327115611944
• 国立国会図書館永続的識別子: info:ndljp/pid/10402625; https://dl.ndl.go.jp/pid/10402625
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2017-08-02T04:31:34+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10564/3184
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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