本資料は、掲載誌(URI)等のリンク先にある学位授与機関のWebサイトやCiNii Dissertationsから、本文を自由に閲覧できる場合があります。
博士論文
国立国会図書館館内限定公開
収録元データベースで確認する
国立国会図書館デジタルコレクション
デジタルデータあり
Studies on DOCK family protein Sponge during Drosophila development
- 国立国会図書館永続的識別子
- info:ndljp/pid/10402908
国立国会図書館での利用に関する注記
資料に関する注記
一般注記:
- The Sponge (Spg) belongs to DOCK family that is widely-conserved among species, and DOCK family members are known as DOCK1?DOCK11 in mammals. DOCK1 in...
書店で探す
障害者向け資料で読む
書店で探す
障害者向け資料で読む
書誌情報
この資料の詳細や典拠(同じ主題の資料を指すキーワード、著者名)等を確認できます。
デジタル
- 資料種別
- 博士論文
- 著者・編者
- Eguchi, Koichi
- 出版年月日等
- 2015-03-25
- 出版年(W3CDTF)
- 2015-03-25
- 並列タイトル等
- ショウジョウバエ発生過程におけるDOCKファミリータンパク質Spongeの研究
- 授与機関名
- 京都工芸繊維大学
- 授与年月日
- 2015-03-25
- 授与年月日(W3CDTF)
- 2015-03-25
- 報告番号
- 甲第730号
- 学位
- 博士(学術)
- 博論授与番号
- 14303甲第730号
- 本文の言語コード
- en
- 一般注記
- The Sponge (Spg) belongs to DOCK family that is widely-conserved among species, and DOCK family members are known as DOCK1?DOCK11 in mammals. DOCK1 involves neurite elongation, DOCK2 involves immunocyte differentiation. However function of other DOCK family member is yet unknown. To carry out further functional analysis of DOCK family, I searched a novel DOCK gene in genome database of Drosophila, and identified the spg gene that is similar to DOCK3 and DOCK4. I focused on this spg gene and established UAS-spgIR transgenic fly strains for knockdown of spg. Specific knockdown of spg by GMR-GAL4 driver in eye imaginal discs induced abnormal compound eye in adults. To examine development of photoreceptor cells and localization of Spg, I crossed this knockdown strains with a variety of enhancer trap strains that specifically expresses lacZ in various set of photoreceptor cells. Immunostaining with anti-Spg and anti-lacZ antibodies revealed high expression of Spg in R7 photoreceptor cells that was specifically reduced by its knockdown with the GMR-GAL4 driver. Furthermore, immunostaining with anti-dpERK antibodies revealed that the level of activated ERK signal was extensively reduced by knockdown of spg with the GMR-GAL4 driver in eye discs. In other tissues, specific knockdown of spg by pannir-GAL4 or apterous-GAL4 driver in wing discs induced split thorax phenotype in adults. Reduction of the Drosophila c-Jun N-terminal kinase (JNK), basket (bsk) gene dose enhanced the spg knockdown-induced phenotype. Monitoring JNK activity in the wing imaginal discs by immunostaining with anti-phosphorylated JNK antibodies revealed the strong reduction of the JNK activity in the spg knockdown clones. Furthermore, the Duolink in situ Proximity Ligation Assay method detected interaction signals between Spg and Rap1 in the eye discs and those between Spg and Rac1 in the wing discs, respectively. Thus Spg that interact Rap1 or Rac1 appears to play a key role in differentiation of tissues by activating ERK or JNK pathway.
- 国立国会図書館永続的識別子
- info:ndljp/pid/10402908
- コレクション(共通)
- コレクション(障害者向け資料:レベル1)
- コレクション(個別)
- 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
- 収集根拠
- 博士論文(自動収集)
- 受理日(W3CDTF)
- 2017-08-02T04:31:34+09:00
- 記録形式(IMT)
- application/pdf
- オンライン閲覧公開範囲
- 国立国会図書館内限定公開
- デジタル化資料送信
- 図書館・個人送信対象外
- 遠隔複写可否(NDL)
- 可
- 掲載誌(URI)
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館デジタルコレクション