DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes

• 資料種別: 博士論文
• ISSN: 1932-6203
• タイトル: DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes
• 巻次・部編番号: 11 8
• 著者・編者: Kuroda, Masashi; Tominaga, Ayako; Nakagawa, Kasumi; Nishiguchi, Misa; Sebe, Mayu; Miyatake, Yumiko; Kitamura, Tadahiro; Tsutsumi, Rie; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi
• 著者標目: Kuroda, Masashi; Tominaga, Ayako; Nakagawa, Kasumi; Nishiguchi, Misa; Sebe, Mayu; Miyatake, Yumiko; Kitamura, Tadahiro; Tsutsumi, Rie; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi
• 出版事項: PLOS
• 出版年月日等: 2016-08-05
• 出版年（W3CDTF）: 2016-08-05
• 並列タイトル等: DNAメチル化は3T3-L1脂肪細胞におけるレプチン発現を抑制する; DNA Methylation Suppresses Leptin Expression in Adipocytes
• タイトル（掲載誌）: PLOS ONE
• 授与機関名: 徳島大学
• 授与年月日: 2017-03-23
• 授与年月日（W3CDTF）: 2017-03-23
• 報告番号: 甲第3087号
• 学位: 博士（栄養学）
• 博論授与番号: 甲第3087号
• 本文の言語コード: eng
• 件名標目: Adipocyte; Leptin; DNA methylation; 3T3-L1; obesity
• 対象利用者: 一般
• 一般注記: Leptin is a key regulator of energy intake and expenditure. This peptide hormone is expressed in mouse white adipose tissue, but hardly expressed in 3T3-L1 adipocytes. Using bisulfite sequencing, we found that CpG islands in the leptin promoter are highly methylated in 3T3-L1cells. 5-azacytidine, an inhibitor of DNA methyltransferase, markedly increased leptin expression as pre-adipocytes matured into adipocytes. Remarkably, leptin expression was stimulated by insulin in adipocytes derived from precursor cells exposed to 5-azacytidine, but suppressed by thiazolidinedione and dexamethasone. In contrast, adipocytes derived from untreated precursor cells were unresponsive to both 5-azacytidine and hormonal stimuli, although lipid accumulation was sufficient to boost leptin expression in the absence of demethylation. Taken together, the results suggest that leptin expression in 3T3-L1 cells requires DNA demethylation prior to adipogenesis, transcriptional activation during adipogenesis, and lipid accumulation after adipogenesis.
• DOI: info:doi/10.1371/journal.pone.0160532; https://doi.org/info:doi/10.1371/journal.pone.0160532
• 国立国会図書館永続的識別子: info:ndljp/pid/10993566; https://dl.ndl.go.jp/pid/10993566
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2017-12-04T02:02:48+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/110107
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp