Generation of fluorescent flowers exhibiting strong fluorescence by combination of fluorescent protein from marine plankton and recent genetic tools in Torenia fournieri Lind.
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DOI[10.5511/plantbiotechnology.14.0907a]のデータに遷移します
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- 資料種別
- 記事
- 著者・編者
- Katsutomo SasakiKo KatoHiroshi Mishima
- 出版年月日等
- 2014
- 出版年（W3CDTF）
- 2014
- タイトル（掲載誌）
- Plant biotechnology
- 巻号年月日等（掲載誌）
- 31(4)
- 掲載巻
- 31(4)
- ISSN（掲載誌）
- 1347-6114
- ISSN-L（掲載誌）
- 1342-4580
- 本文の言語コード
- eng
- DOI
- 10.5511/plantbiotechnology.14.0907a
- 国立国会図書館永続的識別子
- info:ndljp/pid/11000377
- コレクション（共通）
- コレクション（障害者向け資料：レベル1）
- コレクション（個別）
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- 収集根拠
- インターネット資料収集保存事業（WARP）
- 受理日（W3CDTF）
- 2017-12-08T10:56:38+09:00
- 保存日（W3CDTF）
- 2015-08-15
- 記録形式（IMT）
- application/pdf
- オンライン閲覧公開範囲
- インターネット公開
- 遠隔複写可否（NDL）
- 不可
- 掲載誌（国立国会図書館永続的識別子）
- info:ndljp/pid/11000374
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館デジタルコレクション
- コレクション（個別）
- 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会
- オンライン閲覧公開範囲
- インターネット公開
- 遠隔複写可否（NDL）
- 不可
- 所蔵機関
- 国立国会図書館
- 請求記号
- Z54-J126
- 関連情報（国立国会図書館永続的識別子）
- info:ndljp/pid/11000377
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館雑誌記事索引
- 書誌ID（NDLBibID）
- 026003744
- 整理区分コード
- 632
- 要約等
- Florescent proteins have been popularly used for studying genes and proteins of interest in various experiments at a cellular level, such as the analysis of intracellular localization and protein–protein interaction. However, the strength of fluorescence was insufficient for macro level observations of tissues or of the whole plant, and the fluorescent flowers that have been generated so far needed high-sensitive imaging equipment for the observation. Here we generated fluorescent <i>Torenia</i> flowers by the combined use of a high-performance fluorescent protein and the latest protein expression technologies, leading to the production of fluorescent proteins that can be easily and clearly observed. A coding sequence of a yellowish green fluorescent protein from the marine plankton <i>Chiridius poppei</i> (<i>CpYGFP</i>) was fused to the optimized sequences of the heat shock protein terminator and the 5′-untranslated region of the alcohol dehydrogenase gene of <i>Arabidopsis</i> to gain massive accumulation of the fluorescent protein. Strong fluorescence of CpYGFP was apparent in every part of the transgenic plant under the simple combination of a blue LED for excitation and an orange colored transparent acrylic filter for emission, while faint autofluorescence remained in the wild-type plants. By evaluating the combination of excitation wavelengths (excitation and emission filters) we were able to eliminate this undesired fluorescence. The fluorescent flowers could be used for ornamental purposes as well as for the analysis of fluorescent transgenic plants spatiotemporally in a nondestructive manner.
- DOI
- 10.5511/plantbiotechnology.14.0907a
- オンライン閲覧公開範囲
- インターネット公開
- 連携機関・データベース
- 科学技術振興機構 : J-STAGE
- 要約等
- Florescent proteins have been popularly used for studying genes and proteins of interest in various experiments at a cellular level, such as the analysis of intracellular localization and protein–protein interaction. However, the strength of fluorescence was insufficient for macro level observations of tissues or of the whole plant, and the fluorescent flowers that have been generated so far needed high-sensitive imaging equipment for the observation. Here we generated fluorescent <i>Torenia</i> flowers by the combined use of a high-performance fluorescent protein and the latest protein expression technologies, leading to the production of fluorescent proteins that can be easily and clearly observed. A coding sequence of a yellowish green fluorescent protein from the marine plankton <i>Chiridius poppei</i> (<i>CpYGFP</i>) was fused to the optimized sequences of the heat shock protein terminator and the 5′-untranslated region of the alcohol dehydrogenase gene of <i>Arabidopsis</i> to gain massive accumulation of the fluorescent protein. Strong fluorescence of CpYGFP was apparent in every part of the transgenic plant under the simple combination of a blue LED for excitation and an orange colored transparent acrylic filter for emission, while faint autofluorescence remained in the wild-type plants. By evaluating the combination of excitation wavelengths (excitation and emission filters) we were able to eliminate this undesired fluorescence. The fluorescent flowers could be used for ornamental purposes as well as for the analysis of fluorescent transgenic plants spatiotemporally in a nondestructive manner.
- DOI
- 10.5511/plantbiotechnology.14.0907a
- オンライン閲覧公開範囲
- インターネット公開
- 関連情報（URI）
- 参照
- Usefulness of deep-ocean water pumping for the seasonal monitoring of mesozooplanktonDistribution of cell layers in floral organs of chrysanthemum analyzed with periclinal chimeras carrying a transgene encoding fluorescent proteinDissecting promoter of InMYB1 gene showing petal-specific expressionProduction of chrysanthemum periclinal chimeras through shoot regeneration from leaf explantsものづくりの変容<br>二次代謝産物代謝工学の産業利用に関する将来展望Chimerism of chrysanthemum stems changes at the nodes during vegetative growthLettuce-based production of an oral vaccine against porcine edema disease for the seed lot system
- 参照
- Flavonols: old compounds for old rolesThe green fluorescent protein (GFP) as a vital screenable marker in rice transformationDevelopment and Use of Fluorescent Protein Markers in Living CellsThe 5′-untranslated region of the tobacco alcohol dehydrogenase gene functions as an effective translational enhancer in plantThe HSP Terminator of Arabidopsis thaliana Induces a High Level of Miraculin Accumulation in Transgenic TomatoesA Computational and Experimental Approach Reveals that the 5′-Proximal Region of the 5′-UTR has a Cis-Regulatory Signature Responsible for Heat Stress-Regulated mRNA Translation in ArabidopsisTHE GREEN FLUORESCENT PROTEINLocalization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue lightThe 5′-untranslated region of the Oryza sativa alcohol dehydrogenase gene functions as a translational enhancer in monocotyledonous plant cellsThe Fluorescent Toolbox for Assessing Protein Location and FunctionPhotoactivatable mCherry for high-resolution two-color fluorescence microscopyVisualization of autophagy in Arabidopsis using the fluorescent dye monodansylcadaverine and a GFP‐AtATG8e fusion proteinIn Vivo Intracellular pH Measurements in Tobacco and<i>Arabidopsis</i>Reveal an Unexpected pH Gradient in the Endomembrane SystemGFP tagging sheds light on protein translocation: implications for key methods in cell biologyThe HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant CellsCharacterisation of the 5′-leader sequence of tobacco mosaic virus RNA as a general enhancer of translation in vitroImproved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinUse of the GFP Reporter as a Vital Marker for Agrobacterium-Mediated Transformation of Sugar Beet (Beta vulgaris L.)Extraction, Purification and Properties of Aequorin, a Bioluminescent Protein from the Luminous Hydromedusan, <i>Aequorea</i>Post-transcriptional regulation in higher eukaryotes: The role of the reporter gene in controlling expressionDetermination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP->YFP->mRFP FRET detected by flow cytometryFunctional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri LindImaging protein molecules using FRET and FLIM microscopyProton channels in algae: reasons to be excitedStructural basis for red‐shifted emission of a GFP‐like protein from the marine copepod <i>Chiridius poppei</i>Sorting of proteins to storage vacuoles: how many mechanisms?EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversionFRET imagingRecent Developments of Biological Reporter Technology for Detecting Gene ExpressionLinking chlorophyll a fluorescence to photosynthesis for remote sensing applications: mechanisms and challengesA novel yellowish-green fluorescent protein from the marine copepod, Chiridius poppei, and its use as a reporter protein in HeLa cellsEfficient Promoter Cassettes for Enhanced Expression of Foreign Genes in Dicotyledonous and Monocotyledonous PlantsEnergy transfer in a bioluminescent systemIntermolecular energy transfer in the bioluminescent system of AequoreaTautomerism of flavonol glucosides: relevance to plant UV protection and flower colourEngineering of the Rose Flavonoid Biosynthetic Pathway Successfully Generated Blue-Hued Flowers Accumulating DelphinidinThe usefulness of the gfp reporter gene for monitoring Agrobacterium-mediated transformation of potato dihaploid and tetraploid genotypesPhotoactivation and Imaging of Optical Highlighter Fluorescent ProteinsProject review: Beyond the blue rose: modification of floral architecture with plant-specific chimeric repressorsImproved translation efficiency in chrysanthemum and torenia with a translational enhancer derived from the tobacco alcohol dehydrogenase geneThe longer version of Arabidopsis thaliana heat shock protein 18.2 gene terminator contributes to higher expression of stably integrated transgenes in cultured tobacco cellsTrehalose drastically extends the in vitro vegetative culture period and facilitates maintenance of Torenia fournieri plantsUtilization of a floral organ-expressing AP1 promoter for generation of new floral traits in Torenia fournieri LindTorenia fournieri (torenia) as a model plant for transgenic studiesアグロバクテリウムを用いたトレニア形質転換体の作出トレニア属の種間交雑親和性と系統分類との関係
- 連携機関・データベース
- 国立情報学研究所 : CiNii Research
- 提供元機関・データベース
- Japan Link Center雑誌記事索引データベース雑誌記事索引データベースCrossrefCiNii ArticlesCiNii ArticlesCrossrefCrossrefCrossrefCrossrefCrossrefCrossrefCrossref
- 書誌ID（NDLBibID）
- 02600374411000377
- NII論文ID
- 13000470163240020313500