Live-imaging evaluation of the efficacy of elevated CO2 concentration in a closed cultivation system for the improvement of bioproduction in tomato fruits

• 資料種別: 記事
• タイトル: Live-imaging evaluation of the efficacy of elevated CO2 concentration in a closed cultivation system for the improvement of bioproduction in tomato fruits
• 著者・編者: Haruaki Yamazaki; Nobuo Suzui; Yong-Gen Yin
• 出版事項: 日本 : 日本植物細胞分子生物学会
• 出版年月日等: 2015
• 出版年（W3CDTF）: 2015
• タイトル（掲載誌）: Plant biotechnology
• 巻号年月日等（掲載誌）: 32(1)
• 掲載巻: 32(1)
• ISSN（掲載誌）: 1347-6114
• ISSN-L（掲載誌）: 1342-4580
• 本文の言語コード: eng
• DOI: 10.5511/plantbiotechnology.14.1210a; https://doi.org/10.5511/plantbiotechnology.14.1210a
• 国立国会図書館永続的識別子: info:ndljp/pid/11000415; https://dl.ndl.go.jp/pid/11000415
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会; https://dl.ndl.go.jp/collections/B00043
• 収集根拠: インターネット資料収集保存事業（WARP）
• 受理日（W3CDTF）: 2017-12-08T10:56:38+09:00
• 保存日（W3CDTF）: 2015-08-15
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: インターネット公開
• 遠隔複写可否（NDL）: 不可
• 掲載誌（URI）: https://warp.da.ndl.go.jp/info:ndljp/pid/9483390/www.jspcmb.jp/journal/pb32_1.html
• 掲載誌（国立国会図書館永続的識別子）: info:ndljp/pid/11000411; https://dl.ndl.go.jp/pid/11000411
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > 学術機関 > 学協会; https://dl.ndl.go.jp/collections/B00043
• オンライン閲覧公開範囲: インターネット公開
• 遠隔複写可否（NDL）: 不可
• 所蔵機関: 国立国会図書館
• 請求記号: Z54-J126
• 関連情報（URI）: http://dl.ndl.go.jp/info:ndljp/pid/11000415
• 関連情報（国立国会図書館永続的識別子）: info:ndljp/pid/11000415; http://dl.ndl.go.jp/info:ndljp/pid/11000415
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 026292485; http://id.ndl.go.jp/bib/026292485
• 整理区分コード: 632

• 要約等: To maximize fruit yield of tomatoes cultivated in a controlled, closed system such as a greenhouse or a plant factory at a limited cost, it is important to raise the translocation rate of fixed carbon to fruits by tuning the cultivation conditions. Elevation of atmospheric CO₂ concentration is a good candidate; however, it is technically difficult to evaluate the effect on fruit growth by comparing different individuals in different CO₂ conditions because of large inter-individual variations. In this study, we employed a positron-emitting tracer imaging system (PETIS), which is a live-imaging technology for plant studies, and a short-lived radioisotope ¹¹C to quantitatively analyze immediate responses of carbon fixation and translocation in tomatoes in elevated CO₂ conditions. We also developed a closed cultivation system to feed a test plant with CO₂ at concentrations of 400, 1,500 and 3,000 ppm and a pulse of ¹¹CO₂. As a result, we obtained serial images of ¹¹C fixation by leaves and subsequent translocation into fruits. Carbon fixation was enhanced steadily by increasing the CO₂ concentration, but the amount translocated into fruits saturated at 1,500 ppm on average. The translocation rate had larger inter-individual variation and showed less consistent responses to external CO₂ conditions compared with carbon fixation. Our experimental system was demonstrated to be a valuable tool for the optimization of closed cultivation systems because it can trace the responses of carbon translocation in each individual, which are otherwise usually masked by inter-individual variation.
• DOI: 10.5511/plantbiotechnology.14.1210a
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: To maximize fruit yield of tomatoes cultivated in a controlled, closed system such as a greenhouse or a plant factory at a limited cost, it is important to raise the translocation rate of fixed carbon to fruits by tuning the cultivation conditions. Elevation of atmospheric CO₂ concentration is a good candidate; however, it is technically difficult to evaluate the effect on fruit growth by comparing different individuals in different CO₂ conditions because of large inter-individual variations. In this study, we employed a positron-emitting tracer imaging system (PETIS), which is a live-imaging technology for plant studies, and a short-lived radioisotope ¹¹C to quantitatively analyze immediate responses of carbon fixation and translocation in tomatoes in elevated CO₂ conditions. We also developed a closed cultivation system to feed a test plant with CO₂ at concentrations of 400, 1,500 and 3,000 ppm and a pulse of ¹¹CO₂. As a result, we obtained serial images of ¹¹C fixation by leaves and subsequent translocation into fruits. Carbon fixation was enhanced steadily by increasing the CO₂ concentration, but the amount translocated into fruits saturated at 1,500 ppm on average. The translocation rate had larger inter-individual variation and showed less consistent responses to external CO₂ conditions compared with carbon fixation. Our experimental system was demonstrated to be a valuable tool for the optimization of closed cultivation systems because it can trace the responses of carbon translocation in each individual, which are otherwise usually masked by inter-individual variation.
• DOI: 10.5511/plantbiotechnology.14.1210a; https://doi.org/10.5511/plantbiotechnology.14.1210a
• オンライン閲覧公開範囲: インターネット公開
• 関連情報（URI）: http://id.ndl.go.jp/bib/026292485; https://ndlsearch.ndl.go.jp/books/R000000004-I026292485; https://dl.ndl.go.jp/pid/11000415; http://id.ndl.go.jp/digimeta/11000415
• 参照: Visualising spatio-temporal distributions of assimilated carbon translocation and release in root systems of leguminous plants; https://cir.nii.ac.jp/crid/1360013168778985344; Translocation of 11C-labelled photosynthates to strawberry fruits depends on leaf transpiration during twilight; https://cir.nii.ac.jp/crid/1360299456218850048; Noninvasive imaging of hollow structures and gas movement revealed the gas partial‐pressure‐gradient‐driven long‐distance gas movement in the aerenchyma along the leaf blade to submerged organs in rice; https://cir.nii.ac.jp/crid/1360576118684575872; Development of a compact multiprobe system for monitoring positron-emitting tracers in plant stems; https://cir.nii.ac.jp/crid/1360588379366719232; Dynamic Analysis of Photosynthate Translocation Into Strawberry Fruits Using Non-invasive 11C-Labeling Supported With Conventional Destructive Measurements Using 13C-Labeling; https://cir.nii.ac.jp/crid/1360848664393914496; Rice immediately adapts the dynamics of photosynthates translocation to roots in response to changes in soil water environment; https://cir.nii.ac.jp/crid/1360861707144732800; On-line rapid purification of [13N]N2 gas for visualization of nitrogen fixation and translocation in nodulated soybean; https://cir.nii.ac.jp/crid/1361131416546027392; Non-invasive 11C-Imaging Revealed the Spatiotemporal Variability in the Translocation of Photosynthates Into Strawberry Fruits in Response to Increasing Daylight Integrals at Leaf Surface; https://cir.nii.ac.jp/crid/1363388843946494592; 植物科学における放射線イメージング; https://cir.nii.ac.jp/crid/1390001277349028096; Positron-emitting Tracer Imaging System （PETIS） 法を用いたトマト果実への光合成産物の転流に果実直下の側枝葉が及ぼす影響の評価; https://cir.nii.ac.jp/crid/1390567172582877440
• 参照: What determines carbon partitioning between competing sinks?; https://cir.nii.ac.jp/crid/1360011143840765312; CO₂-Enhanced Yield and Foliar Deformation among Tomato Genotypes in Elevated CO₂ Environments; https://cir.nii.ac.jp/crid/1360292619004791808; Real-time whole-plant imaging of 11C translocation using positron-emitting tracer imaging system; https://cir.nii.ac.jp/crid/1360292620713557504; Photoassimilation, Assimilate Translocation and Plasmodesmal Biogenesis in the Source Leaves of Arabidopsis thaliana Grown Under an Increased Atmospheric CO2 Concentration; https://cir.nii.ac.jp/crid/1360567184692679424; A trial of production of the plant-derived high-value protein in a plant factory; https://cir.nii.ac.jp/crid/1360567189912566656; Tracing Cadmium from Culture to Spikelet: Noninvasive Imaging and Quantitative Characterization of Absorption, Transport, and Accumulation of Cadmium in an Intact Rice Plant; https://cir.nii.ac.jp/crid/1360855570690785792; Use of positron-emitting tracer imaging system for measuring the effect of salinity on temporal and spatial distribution of 11C tracer and coupling between source and sink organs; https://cir.nii.ac.jp/crid/1361418520552126464; A compact planar positron imaging system; https://cir.nii.ac.jp/crid/1361981469171431680; Photosynthesis and Plant Growth at Elevated Levels of CO2; https://cir.nii.ac.jp/crid/1363107369795390848; Quantitative Modeling of Photoassimilate Flow in an Intact Plant Using the Positron Emitting Tracer Imaging System (PETIS); https://cir.nii.ac.jp/crid/1363388843382877824; New understanding on phloem physiology and possible consequences for modelling long‐distance carbon transport; https://cir.nii.ac.jp/crid/1363951795814921088; Salinity induces carbohydrate accumulation and sugar-regulated starch biosynthetic genes in tomato (Solanum lycopersicum L. cv. ‘Micro-Tom’) fruits in an ABA- and osmotic stress-independent manner; https://cir.nii.ac.jp/crid/1364233271288129408
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp; Crossref; https://www.crossref.org; CiNii Articles; 科学研究費助成事業データベース; https://kaken.nii.ac.jp/ja
• 書誌ID（NDLBibID）: 026292485; http://id.ndl.go.jp/bib/026292485; 11000415; http://id.ndl.go.jp/bib/11000415
• NII論文ID: 130005061603