肥満2型糖尿病モデルSDT fattyラットの骨格筋についての病態生理学的解析

• 資料種別: 博士論文
• タイトル: 肥満2型糖尿病モデルSDT fattyラットの骨格筋についての病態生理学的解析
• 著者・編者: 剣持, 佑介
• 出版事項: 新潟大学
• 出版年月日等: 2018-03-23
• 出版年（W3CDTF）: 2018-03-23
• 並列タイトル等: 肥満2型糖尿病モデルSDT fattyラットの骨格筋についての病態生理学的解析
• 授与機関名: 新潟大学
• 授与年月日: 2018-03-23
• 授与年月日（W3CDTF）: 2018-03-23
• 報告番号: 甲第4476号
• 学位: 博士（農学）
• 博論授与番号: 甲第4476号
• 本文の言語コード: jpn
• 一般注記: Sarcopenia is the age-related decrease of muscle mass and function. Diabetes and obese are known to be risk factors to exacerbate sarcopenia but the underlying mechanism of diabetes-related sarcopenia is still unknown. An obese type 2 diabetes SDT fatty rat shows an early onset of severe diabetes and there have been no reports on the characteristics of their skeletal muscle. Therefore, pathophysiological analyses were performed for the skeletal muscle in this rat. Diabetic male SDT fatty rats were sacrificed at 8, 16, 24, 32 and 40 weeks of age. Age-matched Sprague-Dawley (SD) rats were used as the normal control. In addition to biological blood parameters, the soleus and the extensor digitorum longus muscles were examined for muscle weight, histopathology and protein synthesis and degradation. Muscle grip strength was also examined. As results, muscle weights of the SDT fatty rats were significantly decreased from 16 weeks of age. The mean cross-sectional area of muscle fibers in the SDT fatty rat decreased from 24 weeks of age. Increased intramyocellular lipid accumulation was observed in the SDT fatty rats from 8 weeks of age, identified by immunohistochemistry for adipophilin and TEM. Plasma insulin-like growth factor (IGF)-1 levels and muscle strength in the SDT fatty rats decreased at 24 weeks of age thereafter. These pathophysiological findings have been reported both in the sarcopenia in aged humans and in patients with diabetes. This study is the first report of skeletal muscle pathology in SDT fatty rats. SDT fatty rats showed severe metabolic disorders including hyperglycemia and hyperlipidemia at younger age than other diabetic rat models and was accompanied by decreases in muscle weight, muscle strength, muscle fiber area and protein synthesis hormone (IGF-1), an increase in IMCL and abnormal mitochondrial morphology. These results suggested that the sarcopenia secondary to impaired glycolipid metabolism was observed in the skeletal muscle of the SDT fatty rats. There have been little reports of comprehensive pathophysiological analyses of the skeletal muscle using diabetic rodent models. In conclusion, SDT fatty rat was considered to be a useful model for analysis of diabetes-related sarcopenia. Details of changes of muscle fiber type proportion, mitochondrial oxidative activity and regenerative potential in skeletal muscle in SDT fatty rats are yet to be investigated. Further investigation of skeletal muscle in the SDT fatty rat is needed.; 学位の種類: 博士（農学）. 報告番号: 甲第4476号. 学位記番号: 新大院博（農）甲第179号. 学位授与年月日: 平成30年3月23日; 新大院博（農）甲第179号; 開始ページ : 1; 終了ページ : 70
• 国立国会図書館永続的識別子: info:ndljp/pid/11123216; https://dl.ndl.go.jp/pid/11123216
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2018-08-03T23:36:01+09:00
• 作成日（W3CDTF）: 2019-08-05
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10191/50314
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp