並列タイトル等STUDY OF LONG-RANGE SURFACE PLASMON RESONANCE BIOSENSORS FOR ABO BLOOD TYPING
ABO 式血液型測定のための長距離伝搬表面プラズモン共鳴バイオセンサーに関する研究
一般注記In this study, I would like to propose new techniques for blood grouping application. Due to the advantage of optical biosensors such as high sensitivity, specificity, simple setup and rapid detection, they have been applied to a variety of biomolecules. Optical biosensors have also been interested in blood grouping application. In this work, I divided in two parts of feasibility studies which were developed as an optical biosensor for blood grouping based on two techniques. In the first study, I demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR. Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs. The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor. For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index. I determined that the results of blood typing using the LR-SPR biosensor were consistent with the results obtained from the agglutination test. I obtained the lowest detection limits of 1.58 × 10^5 cells/ml for RBC-A and 3.83 × 10^5 cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 10^8 cells/ml). The surface of the biosensor can be efficiently regenerated using 20 mM NaOH. The second study, a transmission surface plasmon resonance (T-SPR) technique for ABO blood typing was proposed based on the immunological interaction between surface antigen of the red blood cell (RBC) and immobilized antibody. The antibody specific to blood group A (Anti-A) was covalently immobilized on carboxylic acid on gold grating surface. The RBC samples were detected on the immobilized anti-A where the blood typing was determined from the change in the intensity of the transmission peak by TSPR technique. The maximum TSPR excitation was at an incident angle 35°. All the results of blood typing was all consistent with the results obtained from agglutination test. In summary, as the performance, sensitivity, simply experimental setup and utility of LR-SPR and T-SPR technique, it can easily be applied for ABO blood group typing.
学位の種類: 博士（工学）. 報告番号: 甲第4469号. 学位記番号: 新大院博（工）甲第476号. 学位授与年月日: 平成30年3月23日
CHAPTER 4: International Journal of Analytical Chemistry. Volume 2016, Article ID 1432781.
新大院博（工）甲第476号
開始ページ : 1
終了ページ : 84
DOIinfo:doi/10.1155/2016/1432781
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