A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis 5

• 資料種別: 博士論文
• ISSN: 1420-4096
• タイトル: A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis
• 巻次・部編番号: 43 5
• 著者・編者: Sasaki, Shohei; Segawa, Hiroko; Hanazaki, Ai; Kirino, Ruri; Fujii, Toru; Ikuta, Kayo; Noguchi, Miwa; Sasaki, Sumire; Koike, Megumi; Tanifuji, Kazuya; Shiozaki, Yuji; Kaneko, Ichiro; Tatsumi, Sawako; Shimohata, Takaaki; Kawai, Yoshichika; Narisawa, Sonoko; Millán, José Luis; Miyamoto, Ken-ichi
• 著者標目: Sasaki, Shohei; Segawa, Hiroko; Hanazaki, Ai; Kirino, Ruri; Fujii, Toru; Ikuta, Kayo; Noguchi, Miwa; Sasaki, Sumire; Koike, Megumi; Tanifuji, Kazuya; Shiozaki, Yuji; Kaneko, Ichiro; Tatsumi, Sawako; Shimohata, Takaaki; Kawai, Yoshichika; Narisawa, Sonoko; Millán, José Luis; Miyamoto, Ken-ichi
• 出版事項: S. Karger AG
• 出版年月日等: 2018-09-10
• 出版年（W3CDTF）: 2018-09-10
• 並列タイトル等: 小腸のリン恒常性における小腸型アルカリホスファターゼ(Akp3)の役割について
• タイトル（掲載誌）: Kidney and Blood Pressure Research
• 授与機関名: 徳島大学
• 授与年月日: 2018-09-27
• 授与年月日（W3CDTF）: 2018-09-27
• 報告番号: 甲第3220号
• 学位: 博士（栄養学）
• 博論授与番号: 甲第3220号
• 本文の言語コード: eng
• 件名標目: Hyperphosphatemia; CKD-MBD; Phosphate transporter
• 対象利用者: 一般
• 一般注記: Background/Aims: Hyperphosphatemia is a serious complication of late-stage chronic kidney disease (CKD). Intestinal inorganic phosphate (Pi) handling plays an important role in Pi homeostasis in CKD. We investigated whether intestinal alkaline phosphatase 3 (Akp3), the enzyme that hydrolyzes dietary Pi compounds, is a target for the treatment of hyperphosphatemia in CKD. Methods: We investigated Pi homeostasis in Akp3 knockout mice (Akp3-/-). We also studied the progression of renal failure in an Akp3-/- mouse adenine treated renal failure model. Plasma, fecal, and urinary Pi and Ca concentration were measured with commercially available kit, and plasma fibroblast growth factor 23, parathyroid hormone, and 1,25(OH)2D3 concentration were measured with ELISA. Brush border membrane vesicles were prepared from mouse intestine using the Ca2+ precipitation method and used for Pi transport activity and alkaline phosphatase activity. In vivo intestinal Pi absorption was measured with oral 32P administration. Results: Akp3-/- mice exhibited reduced intestinal type II sodium-dependent Pi transporter (Npt2b) protein levels and Na-dependent Pi co-transport activity. In addition, plasma active vitamin D levels were significantly increased in Akp3-/- mice compared with wild-type animals. In the adenine-induced renal failure model, Akp3 gene deletion suppressed hyperphosphatemia. Conclusion: The present findings indicate that intestinal Akp3 deletion affects Na+-dependent Pi transport in the small intestine. In the adenine-induced renal failure model, Akp3 is predicted to be a factor contributing to suppression of the plasma Pi concentration.
• DOI: info:doi/10.1159/000493379; https://doi.org/info:doi/10.1159/000493379
• 国立国会図書館永続的識別子: info:ndljp/pid/11217341; https://dl.ndl.go.jp/pid/11217341
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2019-01-04T08:16:43+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/112882
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp