Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats 1

• 資料種別: 博士論文
• ISSN: 1347-8613
• タイトル: Effects of irradiation with narrowband-ultraviolet B on up-regulation of histamine H1 receptor mRNA and induction of apoptosis in HeLa cells and nasal mucosa of rats
• 巻次・部編番号: 138 1
• 著者・編者: Fujii, Tatsuya; Kitamura, Yoshiaki; Mizuguchi, Hiroyuki; Okamoto, Kentaro; Sanada, Nanae; Yamada, Takuya; Sugiyama, Manabu; Michinaga, Shotaro; Kitayama, Mika; Fukui, Hiroyuki; Takeda, Noriaki
• 著者標目: Fujii, Tatsuya; Kitamura, Yoshiaki; Mizuguchi, Hiroyuki; Okamoto, Kentaro; Sanada, Nanae; Yamada, Takuya; Sugiyama, Manabu; Michinaga, Shotaro; Kitayama, Mika; Fukui, Hiroyuki; Takeda, Noriaki
• 出版事項: Elsevier|Japanese Pharmacological Society
• 出版年月日等: 2018-09-10
• 出版年（W3CDTF）: 2018-09-10
• 並列タイトル等: ナローバンドUVBがHeLa細胞とアレルギー性鼻炎モデルラットの鼻粘膜におけるヒスタミンH1受容体遺伝子発現の亢進とアポトーシスの誘導に与える影響
• タイトル（掲載誌）: Journal of Pharmacological Sciences
• 授与機関名: 徳島大学
• 授与年月日: 2018-11-22
• 授与年月日（W3CDTF）: 2018-11-22
• 報告番号: 甲第3246号
• 学位: 博士（医学）
• 博論授与番号: 甲第3246号
• 本文の言語コード: eng
• 件名標目: Allergic rhinitis; apoptosis; histamine H1 receptor gene; narrowband-ultraviolet B; phototherapy
• 対象利用者: 一般
• 一般注記: Narrowband-ultraviolet B (NB-UVB) phototherapy is used for the treatment of atopic dermatitis. Previously, we reported that irradiation with 200 mJ/cm2 of 310 nm NB-UVB suppressed phorbol-12- myristate-13-acetate (PMA)-induced up-regulation of histamine H1 receptor (H1R) gene expression without induction of apoptosis in HeLa cells. However, the effect of NB-UVB irradiation on nasal symptoms is still unclear. Here, we show that low dose irradiation with 310 nm NB-UVB alleviates nasal symptoms in toluene 2,4-diisocyanate (TDI)-sensitized allergy model rats. Irradiation with 310 nm NB-UVB suppressed PMA-induced H1R mRNA up-regulation in HeLa cells dose-dependently at doses of 75-200 mJ/cm2 and reversibly at a dose of 150 mJ/cm2 without induction of apoptosis. While, at doses of more than 200 mJ/cm2, irradiation with 310 nm NB-UVB induced apoptosis. Western blot analysis showed that the suppressive effect of NB-UVB irradiation on H1R gene expression was through the inhibition of ERK phosphorylation. In TDI-sensitized rat, intranasal irradiation with 310 nm NB-UVB at an estimated dose of 100 mJ/cm2 once a day for three days suppressed TDI-induced sneezes and upregulation of H1R mRNA in nasal mucosa without induction of apoptosis. These findings suggest that repeated intranasal irradiation with low dose of NB-UVB could be clinically used as phototherapy of AR.
• DOI: info:doi/10.1016/j.jphs.2018.08.011; https://doi.org/info:doi/10.1016/j.jphs.2018.08.011
• 国立国会図書館永続的識別子: info:ndljp/pid/11239255; https://dl.ndl.go.jp/pid/11239255
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2019-02-03T04:35:56+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/112928
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp