Expression of cytokine-induced neutrophil chemoattractant suppresses tumor necrosis factor alpha expression and thereby prevents the follicles from undergoing atresia and apoptosis

• 資料種別: 博士論文
• ISSN: 1447-0578
• タイトル: Expression of cytokine-induced neutrophil chemoattractant suppresses tumor necrosis factor alpha expression and thereby prevents the follicles from undergoing atresia and apoptosis
• 巻次・部編番号: 16 2
• 著者・編者: Tanaka, Yu; Kuwahara, Akira; Ushigoe, Kenjiro; Yano, Yuya; Taniguchi, Yuka; Yamamoto, Yuri; Matsuzaki, Toshiya; Yasui, Toshiyuki; Irahara, Minoru
• 著者標目: Tanaka, Yu; Kuwahara, Akira; Ushigoe, Kenjiro; Yano, Yuya; Taniguchi, Yuka; Yamamoto, Yuri; Matsuzaki, Toshiya; Yasui, Toshiyuki; Irahara, Minoru
• 出版事項: Wiley|Japan Society for Reproductive Medicine
• 出版年月日等: 2017-03-12
• 出版年（W3CDTF）: 2017-03-12
• 並列タイトル等: サイトカイン誘発好中球化学誘引物質の発現はTNFα発現を抑制することにより卵胞の閉鎖およびアポトーシスから回避させる
• タイトル（掲載誌）: Reproductive Medicine and Biology
• 授与機関名: 徳島大学
• 授与年月日: 2019-03-19
• 授与年月日（W3CDTF）: 2019-03-19
• 報告番号: 乙第2107号
• 学位: 博士（医学）
• 博論授与番号: 乙第2107号
• 本文の言語コード: eng
• 件名標目: Apoptosis; follicle; ovary; ovulation; tumor necrosis factor alpha
• 対象利用者: 一般
• 一般注記: Aim: Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family chemokine, similar to interleukin-8 in rats, and is one of the factors that regulates ovulation. However, the mechanism that regulates atresia of the ovaries postovulation is not clearly defined.Methods: Whether antibody-blocking of CINC/gro can alter the number of ovulated oocytes and modulate neutrophil infiltration was investigated. The effect of the antibody on the level of inflammatory cytokine production and follicular atresia was examined. Apoptosis was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 and Bcl2-associated X (Bax).Results: The anti-CINC/gro antibody treatment decreased the number of ovulated oocytes. The messenger RNA levels of cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL analysis revealed a larger number of apoptotic cells in the antibody group, compared with those in the control group, as well as a significant increase in the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration.Conclusion: These findings suggest that ovulation is accelerated by neutrophil infiltration into the theca layer. The CINC/gro appears to synergize with interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro expression suppresses TNF alpha expression and that CINC/gro expression therefore prevents the follicles from undergoing atresia and apoptosis.
• DOI: info:doi/10.1002/rmb2.12022; https://doi.org/info:doi/10.1002/rmb2.12022
• 国立国会図書館永続的識別子: info:ndljp/pid/11278909; https://dl.ndl.go.jp/pid/11278909
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2019-05-06T10:27:56+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/113212
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp