BMP-7/Smad expression in dedifferentiated Schwann cells during axonal regeneration and upregulation of endogenous BMP-7 following administration of PTH (1-34)

• 資料種別: 博士論文
• タイトル: BMP-7/Smad expression in dedifferentiated Schwann cells during axonal regeneration and upregulation of endogenous BMP-7 following administration of PTH (1-34)
• 著者・編者: Kokubu, Naoki
• 著者標目: Kokubu, Naoki
• 出版事項: 三重大学
• 出版年月日等: 2019-03-25
• 出版年（W3CDTF）: 2019-03-25
• 授与機関名: 三重大学
• 授与年月日: 2019-03-25
• 授与年月日（W3CDTF）: 2019-03-25
• 報告番号: 乙医学第1030号
• 学位: 博士(医学)
• 博論授与番号: 乙医学第1030号
• 本文の言語コード: eng
• 著者別名: コクブ, ナオキ; 國分, 直樹
• 件名標目: bone morphogenetic protein-7; Noggin; parathyroid hormone (PTH) (1-34); peripheral nerve regeneration; Schwann cells
• 対象利用者: 一般
• 一般注記: application/pdf; Purpose: To determine the expression and distribution of bone morphogenetic protein (BMP)-7 and related molecules during peripheral nerve regeneration and to assess whether administration of parathyroid hormone (PTH) drug (1-34) potentiates the intrinsic upregulation of BMP-7/Smad signaling. Methods: The rat sciatic nerves were crushed with an aneurysm clip resulting in axonal degeneration. In the normal nerve, and at 1, 2, 4, and 8 weeks after injury, BMP-7, BMP receptors, p-Smad 1/5/8, and Noggin, the endogenous BMP antagonist, were evaluated. Additionally, the distribution of BMP-7 was assessed by fluorescent double immunostaining. In vitro studies were also performed to examine the effect of BMP-7 and PTH (1-34) administration on rat Schwann cells (SCs). Results: Aneurysm clip made reliable animal modelof the nerve injury with recovery at 8 weeks after the injury. BMP-7/Smad protein and mRNA were significantly upregulated on axon-SCs units at 1 week after injury, and this upregulated expression was maintained for 4 weeks. Besides, significant upregulation of Noggin’s expression was observed on axon-SCs units at 2 weeks after injury.Moreover, fluorescent double immunostaining showed co-localization between expression of BMP-7 and p75NTR during axonal regeneration. In the in vitro study, administration of BMP-7 induced significant proliferation of SCs. Application of PTH (1-34) upregulated BMP-7 on SCs. Discussion/conclusion: BMPs were reported to be involved in protection and recovery after injury as well as in neurogenesis. Our current study showed that BMP/Smad signaling molecules were upregulated on dedifferentiated SCs after peripheral nerve injury and that administration of BMP-7 increased SC viability in vitro. These results suggested that axonal regeneration could be induced via upregulation of endogenous BMP-7 on SCs by PTH (1-34) administration.; 本文/Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, Tsu, Japan; 10p
• DOI: info:doi/10.1177/2309499018812953; https://doi.org/info:doi/10.1177/2309499018812953
• 国立国会図書館永続的識別子: info:ndljp/pid/11378290; https://dl.ndl.go.jp/pid/11378290
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2019-11-04T14:30:53+09:00
• 作成日（W3CDTF）: 2019-10-16
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10076/00018412; https://mie-u.repo.nii.ac.jp/records/12793
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

目次

2023-12-15 再収集