Association of increased renal Cyp24a1 gene expression with low plasma 1,25-dihydroxyvitamin D levels in rats with streptozotocin-induced diabetes 1

• 資料種別: 博士論文
• ISSN: 0912-0009
• タイトル: Association of increased renal Cyp24a1 gene expression with low plasma 1,25-dihydroxyvitamin D levels in rats with streptozotocin-induced diabetes
• 巻次・部編番号: 66 1
• 著者・編者: Tajiri, Mari; Nakahashi, Otoki; Kagawa, Tomohiro; Masuda, Masashi; Ohminami, Hirokazu; Iwano, Masayuki; Takeda, Eiji; Taketani, Yutaka; Yamamoto, Hironori
• 著者標目: Tajiri, Mari; Nakahashi, Otoki; Kagawa, Tomohiro; Masuda, Masashi; Ohminami, Hirokazu; Iwano, Masayuki; Takeda, Eiji; Taketani, Yutaka; Yamamoto, Hironori
• 出版事項: Society for Free Radical Research Japan
• 出版年月日等: 2020-01-01
• 出版年（W3CDTF）: 2020-01-01
• 並列タイトル等: ストレプトゾトシン誘導性糖尿病ラットにおける腎Cyp24a1遺伝子発現上昇と血漿1,25-ジヒドロキシビタミンD濃度低下との関連
• タイトル（掲載誌）: Journal of Clinical Biochemistry and Nutrition
• 授与機関名: 徳島大学
• 授与年月日: 2019-11-28
• 授与年月日（W3CDTF）: 2019-11-28
• 報告番号: 甲第3340号
• 学位: 博士（栄養学）
• 博論授与番号: 甲第3340号
• 本文の言語コード: eng
• 件名標目: vitamin D; CYP24A1; CYP24A1-SV; diabetes; streptozotocin
• 対象利用者: 一般
• 一般注記: Decreases in plasma vitamin D concentrations have been reported in diabetes, although the mechanism involved in this decrease is unclear. Here, we investigated the association between Cyp24a1, a vitamin D catabolic enzyme, and abnormalities in vitamin D metabolism in streptozotocin-induced diabetes rats, an animal model of type 1 diabetes. Plasma 1,25-dihydroxyvitamin D [1,25(OH)2D] levels were significantly lower in streptozotocin-induced diabetes rats and renal Cyp24a1 mRNA expression levels were increased. Western blotting analysis of streptozotocin-induced diabetes rats kidney tissues with anti-CYP24A1 antibody showed a strong signal around 40 kDa, which differs from the predicted 50–55 kDa molecular weight for full-length Cyp24a1 and could represent the Cyp24a1-splicing variant that lacks exons 1 and 2. We observed high levels of renal Cyp24a1-splicing variant mRNA expression in streptozotocin-induced diabetes rats. We also confirmed transcriptional up-regulation of endogenous Cyp24a1 mRNA expression through glucocorticoid receptors by glucocorticoid in opossum kidney proximal cells. Taken together, our results indicated that high Cyp24a1 expression levels may play a role in the decrease of plasma 1,25(OH)2D levels in streptozotocin-induced diabetes rats. High plasma corticosterone levels in diabetes may affect transcriptional regulation to promote increases in Cyp24a1 expression.
• DOI: info:doi/10.3164/jcbn.19-79; https://doi.org/info:doi/10.3164/jcbn.19-79
• 国立国会図書館永続的識別子: info:ndljp/pid/11458342; https://dl.ndl.go.jp/pid/11458342
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2020-03-11T23:38:06+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: https://repo.lib.tokushima-u.ac.jp/114242
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp