Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution

• 資料種別: 博士論文
• ISSN: 1349-6867
• タイトル: Effects of Transplanted Human Cord Blood-Mononuclear Cells on Pulmonary Hypertension in Immunodeficient Mice and Their Distribution
• 巻次・部編番号: 64 1-2
• 著者・編者: Sugano, Mikio; Yoshida, Homare; Kurobe, Hirotsugu; Arase, Hiroki; Kinoshita, Hajime; Kitaichi, Takashi; Sugasawa, Noriko; Nakayama, Soichiro; Maeda, Kazuhisa; Irahara, Minoru; Kitagawa, Tetsuya
• 著者標目: Sugano, Mikio; Yoshida, Homare; Kurobe, Hirotsugu; Arase, Hiroki; Kinoshita, Hajime; Kitaichi, Takashi; Sugasawa, Noriko; Nakayama, Soichiro; Maeda, Kazuhisa; Irahara, Minoru; Kitagawa, Tetsuya
• 出版事項: Faculty of Medicine Tokushima University
• 出版年月日等: 2017-02
• 出版年（W3CDTF）: 2017-02
• 並列タイトル等: 免疫欠損肺高血圧モデルマウスに対するヒト臍帯血由来単核球細胞移植の効果; Human cord blood-MNC transplantation improves PH
• タイトル（掲載誌）: The Journal of Medical Investigation
• 授与機関名: 徳島大学
• 授与年月日: 2019-12-26
• 授与年月日（W3CDTF）: 2019-12-26
• 報告番号: 乙第2109号
• 学位: 博士（医学）
• 博論授与番号: 乙第2109号
• 本文の言語コード: eng
• 件名標目: Pulmonary hypertension; cellular therapy; umbilical cord blood; monocrotaline; mononuclear cells
• 対象利用者: 一般
• 一般注記: Objectives : To investigate the effects of human umbilical cord blood-derived mononuclear cell (hUCB-MNC) transplantation on pulmonary hypertension (PH) induced by monocrotaline (MCT) in immunodeficient mice and their distribution. Methods :MCT was administered to BALB/c Slc-nu/nu mice, and PH was induced in mice 4 weeks later. Fresh hUCB-MNCs harvested from a human donor after her delivery were injected intravenously into those PH mice. The medial thickness of pulmonary arterioles, ratio of right ventricular to septum plus left ventricular weight (RV/S+LV), and ratio of acceleration time to ejection time of pulmonary blood flow waveform (AT/ET) were determined 4 weeks after hUCB-MNC transplantation. To reveal the incorporation into the lung, CMTMR-labeled hUCB-MNCs were observed in the lung by fluorescent microscopy. DiR-labeled hUCB-MNCs were detected in the lung and other organs by bioluminescence images. Results : Medial thickness, RV/S+ LV and AT/ET were significantly improved 4 weeks after hUCB-MNC transplantation compared with those in mice without hUCB-MNC transplantation. CMTMR-positive hUCB-MNCs were observed in the lung 3 hours after transplantation. Bioluminescence signals were detected more strongly in the lung than in other organs for 24 hours after transplantation. Conclusions : The results indicate that hUCB-MNCs are incorporated into the lung early after hUCB-MNC transplantation and improve MCT-induced PH.
• DOI: info:doi/10.2152/jmi.64.43; https://doi.org/info:doi/10.2152/jmi.64.43
• 国立国会図書館永続的識別子: info:ndljp/pid/11478835; https://dl.ndl.go.jp/pid/11478835
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2020-04-06T03:04:11+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/114302
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp