並列タイトル等内耳前庭細胞由来の培養上清を用いた胚性幹細胞から内耳前庭有毛細胞への分化誘導
タイトル（掲載誌）Biochemistry and biophysics reports
一般注記type:Thesis
Vestibular hair cells (V-HCs) in the inner ear have important roles and various functions. When V-HCs are damaged, crippling symptoms, such as vertigo, visual field oscillation, and imbalance, are often seen. Recently, several studies have reported differentiation of embryonic stem (ES) cells, as pluripotent stem cells, to HCs, though a method for producing V-HCs has yet to be established. In the present study, we used vestibular cell conditioned medium (V-CM) and effectively induced ES cells to differentiate into V-HCs. Expressions of V-HC-related markers (Math1, Myosin6, Brn3c, Dnah5) were significantly increased in ES cells cultured in V-CM for 2 weeks, while those were not observed in ES cells cultured without V-CM. On the other hand, the cochlear HC-related marker Lmod3 was either not detected or detected only faintly in those cells when cultured in V-CM. Our results demonstrate that V-CM has an ability to specifically induce differentiation of ES cells into V-HCs.
博士（医学）・甲第743号・令和2年3月16日
© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/BY-NC-ND/4.0/).
identifier:Biochemistry and biophysics reports Vol.19 Article No.100649 (2019 May)
identifier:24055808
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3737
identifier:Biochemistry and biophysics reports, 19: Article No.100649
DOIinfo:doi/10.1016/j.bbrep.2019.100649
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