Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu

• 資料種別: 博士論文
• ISSN: 2076-3417
• タイトル: Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticu
• 巻次・部編番号: 10 16
• 著者・編者: Maetani-Yasui, Miki; Mawatari, Kazuaki; Honjo, Airi; Bui, Thi Kim Ngan; Shimohata, Takaaki; Uebanso, Takashi; Aihara, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• 著者標目: Maetani-Yasui, Miki; Mawatari, Kazuaki; Honjo, Airi; Bui, Thi Kim Ngan; Shimohata, Takaaki; Uebanso, Takashi; Aihara, Mutsumi; Emoto, Takahiro; Akutagawa, Masatake; Kinouchi, Yohsuke; Takahashi, Akira
• 出版事項: MDPI
• 出版年月日等: 2020-08-11
• 出版年（W3CDTF）: 2020-08-11
• 並列タイトル等: 腸炎ビブリオにおけるトランスポゾン変異株を用いた長波長紫外線（UVA）感受性に関連する遺伝子の同定; Identification of Genes Associated with Sensitivity to Ultraviolet A (UVA) Irradiation by Transposon Mutagenesis of Vibrio parahaemolyticus
• タイトル（掲載誌）: Applied Sciences
• 授与機関名: 徳島大学
• 授与年月日: 2020-09-24
• 授与年月日（W3CDTF）: 2020-09-24
• 報告番号: 甲第3462号
• 学位: 博士（栄養学）
• 博論授与番号: 甲第3462号
• 本文の言語コード: eng
• 件名標目: Vibrio parahaemolyticus; ultraviolet; light-emitting diodes; UV-LED; Oxidative stress; light-emitting diode; ultraviolet A
• 対象利用者: 一般
• 一般注記: Ultraviolet (UV) irradiation is used to disinfect water and food and can be classified as UVA (detected at wavelengths 320–400 nm), UVB (280–320 nm), and UVC (<280 nm). We developed a method for UVA sterilization of equipment with a UVA-light-emitting diode (LED); however, a high rate of fluence was needed to promote pathogen inactivation. The aim of this study was to identify genes associated with UVA sensitivity with the goal of improving UVA-LED-mediated bactericidal activity. We constructed a transposon-mutant library of Vibrio parahaemolyticus and selected six mutants with high sensitivity to UVA irradiation. Genes associated with this phenotype include F-type H+-transporting ATPases (atp), as well as those involved in general secretion (gsp), and ubiquinone and terpenoid-quinone biosynthesis (ubi). Gene complementation resulted in decreased sensitivity to UVA-LED. The atp mutants had lower intracellular adenosine triphosphate (ATP) concentrations than the wild-type treatment, with 20 mM L-serine resulting in elevated ATP concentrations and decreased sensitivity to UVA-LED. The gsp mutants exhibited high levels of extracellular protein transport and the ubi mutants exhibited significantly different intracellular concentrations of ubiquinone-8. Taken together, our results suggest that the protein products of the atp, gsp, and ubi genes may regulate sensitivity to UVA irradiation.
• DOI: info:doi/10.3390/app10165549; https://doi.org/info:doi/10.3390/app10165549
• 国立国会図書館永続的識別子: info:ndljp/pid/11570148; https://dl.ndl.go.jp/pid/11570148
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2020-11-10T19:58:22+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/115319
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp