Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering 4

• 資料種別: 博士論文
• ISSN: 1937-3384
• タイトル: Cryopreserved Spontaneous Spheroids from Compact Bone-Derived Mesenchymal Stromal Cells for Bone Tissue Engineering
• 巻次・部編番号: 27 4
• 著者・編者: 董, 宏偉; Dong, Hongwei
• 出版年月日等: 2021
• 出版年（W3CDTF）: 2021
• 並列タイトル等: 骨再生治療を目的とした皮質骨由来間葉系間質細胞によるスフェロイドの凍結保存
• タイトル（掲載誌）: Tissue Engineering Part C:Methods
• 授与機関名: 松本歯科大学
• 授与年月日: 2021-07-14
• 授与年月日（W3CDTF）: 2021-07-14
• 報告番号: 甲第239号
• 学位: 博士（歯学）
• 博論授与番号: 甲第239号
• 本文の言語コード: eng
• 件名標目: Cryopreservation; spontaneous spheroid; osteogenic differentiation capability; compact bone-derived mesenchymal stromal cells,; MSCs
• 一般注記: Spontaneously formed spheroids from mouse compact bone-derived mesenchymal stromal cells (CB-MSCs) possess enhanced stemness and superior plasticity. In this study,the effect of cryopreservation on viabil ity,stemness,and osteogenic differentiation capability of spontaneous CB-MSC spheroids were investigated.CB-MSCs were isolated from mouse femur and tibia. Spheroids were cryopreserved with various concentra tions of dimethyl sulfoxide (DMSO). After thawing,the number of living and dead cells was measured.The expression levels of stem cell markers and osteogenic marker genes were analyzed. The cryopreserved and noncryopreserved spheroids were transplanted in mice with a beta-tricalcium phosphate as a scaffold to evaluate the in vivo bone-forming capability.The percentage of living cells was highest when 5% DMSO was used as a cryoprotectant,confirmed by the number of dead cells.The expression of stem cell marker genes and osteogenic differentiation capability were maintained after cryopreservation with 5% DMSO. The cryopre served spontaneous CB-MSC spheroids showed remarkable new bone formation in vivo,identical to that of the noncryopreserved spheroids even without osteogenic induction. The cryopreserved spontaneous CB-MSC spheroids retained stemness and osteogenic differentiation capability and highlight the utility of spontaneous CB-MSC spheroids as ready-to-use tissue-engineered products for bone tissue engineering.; 2021; identifier:甲第239号; PubMed番号 : 33267737; 元資料の権利情報 : ©️ Hongwei Dong et al.2021; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and re- production in any medium, provided the original work is properly cited.; 元資料の権利情報 : CC BY; 開始ページ : 253; 終了ページ : 263
• 国立国会図書館永続的識別子: info:ndljp/pid/11976672; https://dl.ndl.go.jp/pid/11976672
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-01-10T16:22:37+09:00
• 作成日（W3CDTF）: 2022-01-05
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 原資料（URI）: 雑誌掲載論文(電子版）; https://doi.org/10.1089/ten.tec.2021.0001; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064946/
• 掲載誌（URI）: http://id.nii.ac.jp/1070/00002959/
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp