A newly established monoclonal antibody against ERCC1 detects major isoforms of ERCC1 in gastric cancer

• 資料種別: 博士論文
• ISSN: 2434-9186
• タイトル: A newly established monoclonal antibody against ERCC1 detects major isoforms of ERCC1 in gastric cancer
• 巻次・部編番号: 3 4
• 著者・編者: 大石, 敬之
• 著者標目: 大石, 敬之
• 出版事項: National Center for Global Health and Medicine
• 出版年月日等: 2021-12-01; 3 4
• 出版年（W3CDTF）: 2021-12-01
• 並列タイトル等: 胃癌におけるERCC1の主要なアイソフォームを検出する新たな抗ERCC1モノクローナル抗体の作製
• タイトル（掲載誌）: Global Health & Medicine
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2021-12-01
• 授与年月日（W3CDTF）: 2021-12-01
• 報告番号: 甲医歯薬第1380号
• 学位: 博士(医学)
• 博論授与番号: 甲医歯薬第1380号
• 本文の言語コード: eng
• 件名標目: excision repair cross-complementing group 1; gastric tumor; cisplatin; predictive biomarker
• 対象利用者: 一般
• 一般注記: Identifying patients resistant to cisplatin treatment is expected to improve cisplatin-based chemotherapy for various types of cancers. Excision repair cross-complementing group 1 (ERCC1) is involved in several repair processes of cisplatin-induced DNA crosslinks. ERCC1 overexpression is reported as a candidate prognostic factor and considered to cause cisplatin resistance in major solid cancers. However, anti-ERCC1 antibodies capable of evaluating expression levels of ERCC1 in clinical specimens were not fully optimized. A mouse monoclonal antibody against human ERCC1 was generated in this study. The developed antibody 9D11 specifically detected isoforms of 201, 202, 203 but not 204, which lacks the exon 3 coding region. To evaluate the diagnostic usefulness of this antibody, we have focused on gastric cancer because it is one of the major cancers in Japan. When ERCC1 expression was analyzed in seventeen kinds of human gastric cancer cell lines, all the cell lines were found to express either 201, 202, and/or 203 as major isoforms of ERCC1, but not 204 by Western blotting analysis. Immunohistochemical staining showed that ERCC1 protein was exclusively detected in nuclei of the cells and a moderate level of constant positivity was observed in nuclei of vascular endothelial cells. It showed a clear staining pattern in clinical specimens of gastric cancers. Antibody 9D11 may thus be useful for estimating expression levels of ERCC1 in clinical specimens.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1380号 学位授与年月日:令和3年12月1日; Author: Takayuki Oishi, Yuka Sasaki, Ying Tong, Lichao Chen, Takae Onodera, Satoru Iwasa, Emiko Udo, Bungo Furusato, Hiroaki Fujimori, Shoji Imamichi, Takuya Honda, Tadayoshi Bessho, Junya Fukuoka, Kazuto Ashizawa, Kazuyoshi Yanagihara, Kazuhiko Nakao, Yasuhide Yamada, Nobuyoshi Hiraoka, Mitsuko Masutani; Citation: Global Health & Medicine, 3(4), pp. 226-235; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01)
• DOI: info:doi/10.35772/ghm.2021.01001; https://doi.org/info:doi/10.35772/ghm.2021.01001
• 国立国会図書館永続的識別子: info:ndljp/pid/12154274; https://dl.ndl.go.jp/pid/12154274
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-03-06T04:54:00+09:00
• 作成日（W3CDTF）: 2022-02-15
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: http://hdl.handle.net/10069/00041141
• 掲載誌（URI）: http://hdl.handle.net/10069/00041267
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp