並列タイトル等マイクロアレイ解析に基づいたケロイド遺伝子発現プロファイリングにより、ケロイド病変部におけるMDFIの発現増加を明らかにした
タイトル（掲載誌）Clinical and Experimental Dermatology
授与機関名Nagasaki University (長崎大学)
一般注記Summary Background Keloid lesions are characterized by mesenchymal cell proliferation and excessive extracellular matrix deposition. Previous microarray analyses have been performed to investigate the mechanism of keloid development. However, the molecular pathology that contributes to keloid development remains obscure. Objective Exploring the underlying essential molecules of keloids using microarrays. Methods We performed microarray analyses of keloid and non-lesional skin tissues both in vivo and in vitro. Gene expression levels were compared between tissues and cells. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunopathological staining were used to determine the expression levels of molecules of interest in keloid tissues. Results Several common molecules were upregulated in both keloid tissues and keloid-lesional fibroblasts. PTPRD and NTM were upregulated both in vivo and in vitro. MDFI and ITGA4 were located at the center of the gene co-expression network analysis using keloid tissuess. qRT-PCR revealed significant expression levels of PTPRD and MDFI in keloid tissues. Immunopathological staining revealed that MDFI-positive cells, which have fibroblast characteristics, were located in the keloid-associated lymphoid tissue (KALT) portion of the keloid tissue. Conclusions Our gene expression profiles of keloids distinguished the difference between lesional tissue and cultured lesional fibroblasts, and MDFI was commonly expressed in both tissues and cells. Thus, MDFI-positive cells, which were located in the KALT, might be used in in vitro keloid studies and may play an important role in keloid pathogenesis.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1349号 学位授与年月日:令和3年6月2日
Author: M. Asai, Y. Koike, Y. Kuwatsuka, Y. Yagi, K. Kashiyamam, K. Tanaka, H. Mishima, K. Yoshiura, A. Utani, H. Murota
Citation: Clinical and Experimental Dermatology, Article in Press; 2021
identifier:Nagasaki University (長崎大学), 博士(医学) (2021-06-02)
http://hdl.handle.net/10069/00040832
DOIinfo:doi/10.1111/ced.14698
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