並列タイトル等九州地方の主要国際港における外来ヒトスジシマカの探索
タイトル（掲載誌）PLoS Neglected Tropical Diseases
授与機関名Nagasaki University (長崎大学)
一般注記Background: The Asian tiger mosquito, Aedes albopictus, has spread around the world. The migration was mainly mediated by maritime transportations. This species is known as an efficient vector for arboviruses, and it was responsible for the recent dengue outbreak in Tokyo, Japan. As the vector competence varies among geographical populations, and insecticide resistant populations have emerged, it is important to reveal their movements. The present study uses molecular techniques to search for a sign of introduction of an exotic population in three major international seaports on Kyushu Island.Methodology/principal findings: Adults of Ae. albopictus were sampled around the international seaports of Fukuoka, Kitakyushu, and Nagasaki. Pairwise fixation indexes were estimated between the sampled populations based on 13 microsatellite markers. There was no clear genetic differentiation between distant and port populations in Kitakyushu and Nagasaki. However, the analysis found one distinct group near the container terminal in Fukuoka, which handles international freight containers mainly from adjacent countries. DNA samples were also obtained from Goto, Tsushima, Honshu, Ryukyu, Thailand, and the Philippines; and a cluster analysis and discriminant analysis revealed that the distinct group in Fukuoka did not belong to these groups. Combined with the results of phylogenetic analysis based on CO1, these results implied that this group originated from one Asian temperate region outside of Japan. Neutrality test and mismatch distribution analysis suggested that the establishment of this group was not recent.Conclusions/significance: The present study found a sign of Ae. albopictus introduction from a temperate region of Asia through maritime freight container transportation. The genetically distinct group found in Fukuoka likely originated from a temperate region outside of Japan. Maritime container transportation may introduce to Japan mosquitoes with greater vector competence/insecticide resistance. This is the first study to describe the spatial population structure of Ae. albopictus in Japan using molecular techniques.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1387号 学位授与年月日:令和3年12月1日
Author: Chao Yang, Toshihiko Sunahara, Jinping Hu, Kyoko Futami, Hitoshi Kawada, Noboru Minakawa
Citation: PLoS Neglected Tropical Diseases, 15(10), art. no. e0009827; 2021
identifier:Nagasaki University (長崎大学), 博士(医学) (2021-12-01)
http://hdl.handle.net/10069/00041148
DOIinfo:doi/10.1371/journal.pntd.0009827
コレクション（個別）国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
受理日（W3CDTF）2022-06-05T18:01:14+09:00
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