GHSR methylation‑dependent expression of a variant ligand and receptor of the ghrelin system induces thymoma tumorigenesis

• 資料種別: 博士論文
• ISSN: 1792-1074
• タイトル: GHSR methylation‑dependent expression of a variant ligand and receptor of the ghrelin system induces thymoma tumorigenesis
• 巻次・部編番号: 22 5
• 著者・編者: Tegshee, Bilguun; Kondo, Kazuya; Soejima, Shiho; Muguruma, Kyoka; Tsuboi, Mitsuhiro; Kajiura, Koichiro; Kawakami, Yukikiyo; Kawakita, Naoya; Toba, Hiroaki; Yoshida, Mitsuteru; Takizawa, Hiromitsu; Tangoku, Akira
• 著者標目: Tegshee, Bilguun; Kondo, Kazuya; Soejima, Shiho; Muguruma, Kyoka; Tsuboi, Mitsuhiro; Kajiura, Koichiro; Kawakami, Yukikiyo; Kawakita, Naoya; Toba, Hiroaki; Yoshida, Mitsuteru; Takizawa, Hiromitsu; Tangoku, Akira
• 出版事項: Spandidos Publications
• 出版年月日等: 2021-09-17
• 出版年（W3CDTF）: 2021-09-17
• 並列タイトル等: GHSR遺伝子のメチル化に関連するグレリン系バリアントリガンドと受容体の発現は胸腺腫の腫瘍形成を誘導する; EXPRESSION OF GHRELIN SYSTEM CONSTITUENTS IN THYMIC EPITHELIAL TUMORS
• タイトル（掲載誌）: Oncology Letters
• 授与機関名: 徳島大学
• 授与年月日: 2022-03-23
• 授与年月日（W3CDTF）: 2022-03-23
• 報告番号: 甲第3608号
• 学位: 博士（保健学）
• 博論授与番号: 甲第3608号
• 本文の言語コード: eng
• 件名標目: ghrelin; GHSR; In‑1 ghrelin; thymoma; thymic carcinoma; thymic epithelial tumors
• 対象利用者: 一般
• 一般注記: Our previous study reported that the DNA methylation of growth hormone secretagogue receptor (GHSR) was significantly higher in thymoma or thymic carcinoma (TC) than in normal thymic tissue samples. Thymic epithelial tumors (TETs) with higher GHSR DNA methylation were associated with significantly worse prognosis than those with lower levels of DNA methylation. Diversified components of the ghrelin‑GHSR axis may exert opposing effects in cancer progression, depending on the cancer type in question. However, the precise function of the axis remains unclear. In the present study, the mRNA expression of five key components of the ghrelin system [native ligand ghrelin, variant ligand In‑1 ghrelin, native receptor GHSR1a, variant receptor GHSR1b and acylation enzyme ghrelin O‑acyltransferase (GOAT)] were examined in 58 TET samples by reverse transcription‑quantitative PCR, and protein expression of GHSR1a and GHSR1b was assessed in 20 TETs using immunohistochemistry. The results revealed that In‑1 ghrelin, GHSR1b (variant forms) and GOAT were more strongly expressed in thymoma compared with thymic‑adjacent tissue. By contrast, no significant differences were observed in the expression of ghrelin and GHSR1a (native forms) between thymoma and thymic tissue. The mRNA expression of In‑1 ghrelin and GHSR1b (variant forms) was positively associated with GHSR methylation in thymoma tissue samples. However, a relationship was not found between ghrelin, GHSR1a or GOAT expression (native forms) and GHSR methylation in thymoma. Immunohistochemical analysis revealed that mRNA expression of GHSR1a and GHSR1b generally correlated with expression of the corresponding protein, and that the expression of GHSR1b was increased in advanced‑stage TETs. These results indicate that the DNA methylation of GHSR is associated with a shift from native expression (ghrelin and GHSR1a) to variant expression (In‑1 ghrelin and GHSR1b), which induces the tumorigenesis of thymoma, but not TC.
• DOI: info:doi/10.3892/ol.2021.13054; https://doi.org/info:doi/10.3892/ol.2021.13054
• 国立国会図書館永続的識別子: info:ndljp/pid/12297064; https://dl.ndl.go.jp/pid/12297064
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-06-05T18:01:14+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/116971
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp