53BP1 expression as a biomarker to differentiate thyroid follicular tumors 3

• 資料種別: 博士論文
• タイトル: 53BP1 expression as a biomarker to differentiate thyroid follicular tumors
• 巻次・部編番号: 10 3
• 著者・編者: 佐藤, 綾子
• 著者標目: 佐藤, 綾子
• 出版事項: Bioscientifica Ltd
• 出版年月日等: 2022-03-02; 10 3
• 出版年（W3CDTF）: 2022-03-02
• 並列タイトル等: 甲状腺濾胞性腫瘍における53BP1を用いた術前鑑別の有用性
• タイトル（掲載誌）: Endocrine Connections
• 掲載ページ: 309-315
• ISSN（掲載誌）: 20493614
• 授与機関名: Nagasaki University (長崎大学)
• 授与年月日: 2022-03-02
• 授与年月日（W3CDTF）: 2022-03-02
• 報告番号: 甲医歯薬第1405号
• 学位: 博士(医学)
• 博論授与番号: 甲医歯薬第1405号
• 本文の言語コード: eng
• 件名標目: 53BP1; genomic instability; immunofluorescence; thyroid follicular tumor; liquid-based cytology
• 対象利用者: 一般
• 一般注記: We have previously reported that the expression of p53-binding protein 1 (53BP1) in nuclear foci (NF), a marker reflecting DNA damage response (DDR), detected using immunofluorescence (IF) is useful to estimate the malignant potency of diverse cancers. In this prospective study, we clarified the impact of 53BP1 expression via IF as a biomarker to differentiate thyroid follicular tumors (FTs) with liquid-based cytology (LBC). A total of 183 consecutively obtained-LBC samples, which were preoperatively suspected as FTs, were analyzed. Before histological diagnosis, the type of 53BP1 immunoreactivity in LBC was classified as follows: low DDR type, one or two NF; high DDR type, three or more NF; large foci type, larger than 1.0 μm; abnormal type, intense nuclear staining. Among the 183 cases, 136 cases were postoperatively diagnosed as FTs, including adenomatous goiter (AG, n = 30), follicular adenoma (FA, n = 60), FT-uncertain malignant potency (FT-UMP, n = 18), and follicular carcinoma (FC, n = 28), and 47 cases were diagnosed as tumors other than FTs or technically inadequate materials. Total 136 FT cases were collated with the type of 53BP1 immunoreactivity in LBC. The mean incidence expressing abnormal 53BP1 expression was significantly higher in FC than FA (9.5% vs 2.6%, P-value < 0.001). When adopting 4.3% as a cut-off value to distinguish FC from FA, the sensitivity, specificity, positive predictive value, and negative predictive value were 89.3, 83.3, 71.4, and 94.3%, respectively. Therefore, IF analysis of 53BP1 expression can be employed as a novel technique to diagnose FTs and to distinguish between different types of FTs using LBC.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1405号 学位授与年月日:令和4年3月2日; Author: Ayako Sato, Katsuya Matsuda, Takahiro Motoyama, Zhanna Mussazhanova, Ryota Otsubo, Hisayoshi Kondo, Yuko Akazawa, Miyoko Higuchi, Ayana Suzuki, Mitsuyoshi Hirokawa, Akira Miyauchi, Takeshi Nagayasu, and Masahiro Nakashima; Citation: Endocrine Connections, 10(3), pp. 309–315; 2021; identifier:Nagasaki University (長崎大学), 博士(医学) (2022-03-02); 機関リポジトリ記載の権利情報: © 2021 The authors. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
• 国立国会図書館永続的識別子: info:ndljp/pid/12297071; https://dl.ndl.go.jp/pid/12297071
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-06-05T18:01:14+09:00
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: https://doi.org/10.1530/EC-20-0630
• 掲載誌（URI）: http://hdl.handle.net/10069/00041504
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp