Reduction in Plasmodium falciparum Pfk13 and pfg377 allele diversity through time in southern Vietnam

• 資料種別: 記事
• タイトル: Reduction in Plasmodium falciparum Pfk13 and pfg377 allele diversity through time in southern Vietnam
• 著者・編者: Nguyen Quang Thieu; Vu Duc Chinh; Truong Van Hanh
• 出版事項: 日本 : Springer Nature
• 出版年月日等: 2022-03-01
• 出版年（W3CDTF）: 2022-03-01
• タイトル（掲載誌）: Tropical medicine and health
• 巻号年月日等（掲載誌）: 50(19)
• 掲載巻: 50(19)
• ISSN（掲載誌）: 1349-4147
• ISSN-L（掲載誌）: 1348-8945
• 本文の言語コード: eng
• DOI: 10.1186/s41182-022-00409-4; https://doi.org/10.1186/s41182-022-00409-4
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• 受理日（W3CDTF）: 2022-07-11T22:10:26+09:00
• 保存日（W3CDTF）: 2022-07-11
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• 掲載誌（URI）: http://tropmedhealth.biomedcentral.com/articles
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• 要約等: Background: Plasmodium falciparum has acquired resistance to artemisinin in Southeast Asia, with mutations in the P. falciparum Kelch-13 (Pfk13) gene associated with the resistance phenotype. The widespread use of Artemisinin-based combination therapy (ACT)s in Southeast Asia has led to the selection and spread of parasites carrying mutations in Pfk13. We characterised the allele diversity of Pfk13 and pfg377, an artemisinin-resistance neutral polymorphic gene, in parasite DNA extracted human blood from in southern Vietnam in 2003, 2012, 2015 and 2018. Method: This study was conducted in Bu Gia Map commune, Binh Phuoc province, Vietnam, from May 2018 to January 2019. Twenty-four samples from 2018 to 2019, 30 from 2003, 24 from 2012 and 32 from 2015 were analysed. Malaria-infected human blood was collected by finger-prick and used for molecular analysis. A nested-PCR targeting the small subunit ribosomal RNA gene was used for Plasmodium species identification, followed by amplification and nucleotide sequencing of Pfk13 and region 3 of pfg377. Archived blood samples collected in the same region in 2012 and 2015 were also analysed as above for comparison. Results: The genetic diversity of Pfk13 and pfg377 was lower in 2018–2019 compared to 2012 and 2015. The number of distinct Pfk13 mutants decreased from three in 2012 and 2015, P553L, V568G and C580Y, to one, C580Y in 2018–2019. In 2018–2019, the frequency of C580Y mutant strains was 71% (17/24 isolates). All samples were wild type in 2003. In 2012 and 2015, there were single-strain infections as well as co-infections with two mutant strains or with mutant and wild strains, whereas there were no co-infections in 2018. pfg377 allele diversity decreased from five alleles in 2012 to two alleles in 2018–2019. Conclusion: The genetic diversity of P. falciparum was reduced at the two genetic loci surveyed in this study, Pfk13 and pfg377. In the case of the former gene, we observed an increase in the prevalence of parasites carrying the C580Y gene, known to confer reduced susceptibility to ACTs. The reduction in the diversity of pfg377 may be linked to the clonal expansion of parasite strains carrying the C580Y mutation, leading to an overall reduction in parasite genetic diversity across the population.; Tropical Medicine and Health, 50, art. no. 19; 2022
• DOI: 10.1186/s41182-022-00409-4; https://doi.org/10.1186/s41182-022-00409-4; 10.60692/8k8qz-a0q40; https://doi.org/10.60692/8k8qz-a0q40; 10.60692/daebr-j9t62; https://doi.org/10.60692/daebr-j9t62
• オンライン閲覧公開範囲: インターネット公開
• 著作権情報: © The Author(s) 2022. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
• 関連情報（URI）: http://hdl.handle.net/10069/00041319; https://nagasaki-u.repo.nii.ac.jp/records/27159; http://id.ndl.go.jp/digimeta/12307913; https://dl.ndl.go.jp/pid/12307913; https://link.springer.com/content/pdf/10.1186/s41182-022-00409-4.pdf; https://link.springer.com/article/10.1186/s41182-022-00409-4/fulltext.html; https://search.jamas.or.jp/link/ui/2023194316
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• 書誌ID（NDLBibID）: 12307913; http://id.ndl.go.jp/bib/12307913