Syngeneically transplanted insulin producing cells differentiated from adipose derived stem cells undergo delayed damage by autoimmune responses in NOD mice

• 資料種別: 博士論文
• ISSN: 2045-2322
• タイトル: Syngeneically transplanted insulin producing cells differentiated from adipose derived stem cells undergo delayed damage by autoimmune responses in NOD mice
• 巻次・部編番号: 12
• 著者・編者: Tokuda, Kazunori; Ikemoto, Tetsuya; Yamashita, Shoko; Miyazaki, Katsuki; Okikawa, Shohei; Yamada, Shinichiro; Saito, Yu; Morine, Yuji; Shimada, Mitsuo
• 著者標目: Tokuda, Kazunori; Ikemoto, Tetsuya; Yamashita, Shoko; Miyazaki, Katsuki; Okikawa, Shohei; Yamada, Shinichiro; Saito, Yu; Morine, Yuji; Shimada, Mitsuo
• 出版事項: Springer Nature
• 出版年月日等: 2022-04-07
• 出版年（W3CDTF）: 2022-04-07
• 並列タイトル等: 同系移植された脂肪由来幹細胞から分化したインスリン産生細胞は、NODマウスの自己免疫反応により遅発性の損傷を受ける
• タイトル（掲載誌）: Scientific Reports
• 授与機関名: 徳島大学
• 授与年月日: 2022-09-22
• 授与年月日（W3CDTF）: 2022-09-22
• 報告番号: 甲第3663号
• 学位: 博士（医学）
• 博論授与番号: 甲第3663号
• 本文の言語コード: eng
• 件名標目: insulin producing cell (IPC); 65-kDa isoform of glutamic acid decarboxylase (GAD65); programmed cell death ligand 1 (PD-L1); type 1 diabetes mellitus; non-obese diabetic mouse
• 対象利用者: 一般
• 一般注記: Insulin-producing cells (IPCs) generated by our established protocol have reached the non-clinical ‘proof of concept’ stage. Our strategy for their clinical application is the autotransplantation of IPCs into patients with type 1 diabetes mellitus (T1DM). In this context, the autoimmunity that characterized T1DM is important, rather than allorejection. We aimed to determine how these IPCs respond to T1DM autoimmunity. IPCs were generated from the subcutaneous fat tissue of non-obese diabetic (NOD) mice using our protocol. IPCs derived from NOD mice were transplanted under the kidney capsules of NOD mice at the onset of diabetes and the subsequent changes in blood glucose concentration were characterized. Blood glucose decreased within 30 days of transplantation, but increased again after 40–60 days in three of four recipient NOD mice. In tissue samples, the numbers of CD4+ and CD8+ T cells were significantly higher 60 days after transplantation than 30 days after transplantation. In conclusion, IPCs significantly ameliorate the diabetes of mice in the short term, but are damaged by autoimmunity in the longer term, as evidenced by local T cells accumulation. This study provides new insights into potential stem cell therapies for T1DM.
• DOI: info:doi/10.1038/s41598-022-09838-x; https://doi.org/info:doi/10.1038/s41598-022-09838-x
• 国立国会図書館永続的識別子: info:ndljp/pid/12360867; https://dl.ndl.go.jp/pid/12360867
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2022-11-07T16:56:35+09:00
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/117618
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp