授与機関名Nagasaki University (長崎大学)
一般注記In forensic practice, the presence of chicken fat clots (CFCs) in the heart and/or large blood vessels of cadavers has been empirically used to estimate the time from the onset of fatal events to death. However, little scientific evidence of its significance exists, and the mechanism of its formation has not been elucidated. CFCs contain large amounts of leukocytes; thus, we hypothesized that leukocytes might contribute to their formation. Since leukocytes, especially neutrophils, are considered to be involved in blood coagulation through the formation of neutrophil extracellular traps (NETs), we aimed to investigate whether NETs are related to the formation of CFCs through immunohistochemistry. Most cells in the CFCs were myeloperoxidase- and neutrophil elastase-positive, strongly suggesting that they were neutrophils. Since chromatin is released extracellularly during NET formation, immunostaining was performed against some types of histones in CFCs. A certain number of neutrophils in CFCs showed positive extra-nuclear and extracellular signals of histones. In addition, citrullination of histone H3, which is considered important for histone release, was immunohistochemically detected in some neutrophils. These results suggest that neutrophils may affect the formation of CFCs through histone release. Although it was not clear how and when citrullination and extracellular release of histones in CFCs occur in this study, our findings provide insights into the events occurring at the time of death in a human body.
長崎大学学位論文 学位記番号:博(医歯薬)甲第1491号 学位授与年月日:令和5年3月1日
Author: Maiko Matsuda, Takehiko Murase, Mizuki Komaki, Yoriko Shinba, Masahide Mitsuma, Yuki Abe, Hiromi Yamashita, Nobuhiro Yukawa, Kazuya Ikematsu
Citation: Legal Medicine, 61, art. no. 102208; 2023
identifier:Nagasaki University (長崎大学), 博士(医学) (2023-03-01)
http://hdl.handle.net/10069/00042224
コレクション（個別）国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
受理日（W3CDTF）2023-05-05T22:12:35+09:00
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