並列タイトル等髄床底部への意図的穿孔形成がマウス歯の再植後の歯髄治癒過程に及ぼす影響
一般注記Objectives: Shortening the root of a mouse molar prior to tooth replantation results in early revascularization in the pulp cavity and activation of the dental pulp quiescent stem cells. This study aimed to elucidate the effects of perforating the floor of the pulp chamber on pulpal healing after tooth replantation in order to determine whether this strategy is a valid methodology to promote early revascularization into the pulp. Methods: The maxillary first molars of three-week-old Crlj:CD1 mice were extracted and repositioned into the original socket: the left teeth were immediately replanted (control group: CG), whereas the floor of the pulp chamber of the right teeth was perforated with a tungsten carbide bur before tooth replantation (experimental group: EG). The samples were collected from three days to eight weeks after the operation. In addition to the TUNEL assay, immunohistochemistry for Nestin, CK14, and Ki-67 was conducted. Results: Early revascularization occurred with the decrease of apoptosis and an increase in cell proliferation to facilitate the pulpal healing in the EG compared with the CG. The rate of Nestin-positive perimeter in the distal root significantly increased on days 5 and 14 as well as Nestin-positive hard tissue on day 14. On day 7, the number of epithelial cell rests of Malassez in the EG significantly decreased, making the EG susceptible to ankylosis at the floor. Conclusions: Intentionally perforating the floor of the pulp chamber provides a route via the floor for early revascularization, resulting in the better pulpal healing after tooth replantation.
Journal of Oral Biosciences. 2023, 65(1), 31-39.
新大院博(保)甲第54号
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