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博士論文
国立国会図書館館内限定公開
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国立国会図書館デジタルコレクション
デジタルデータあり(三重大学)
The gut microbiota composition in patients with right-and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing
- 国立国会図書館永続的識別子
- info:ndljp/pid/13024272
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- application/pdfBackground: Gut pathological microbial imbalance or dysbiosis is closely associated with colorectal cancer. Although there are observab...
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デジタル
- 資料種別
- 博士論文
- 著者・編者
- Suga, Daisuke
- 著者標目
- 出版事項
- 出版年月日等
- 2022-12-21
- 出版年(W3CDTF)
- 2022-12-21
- 授与機関名
- 三重大学
- 授与年月日
- 2022-12-21
- 授与年月日(W3CDTF)
- 2022-12-21
- 報告番号
- 甲医学第2164号
- 学位
- 博士(医学)
- 本文の言語コード
- eng
- 対象利用者
- 一般
- 一般注記
- application/pdfBackground: Gut pathological microbial imbalance or dysbiosis is closely associated with colorectal cancer. Although there are observable differences in molecular and clinical characteristics between patients with right- and left-sided colon cancer, differences in their gut microbiomes have not been thoroughly investigated. Furthermore, subsequent changes in microbiota status after partial colectomy remain unknown. We examined the human gut microbiota composition to determine its relationship with colon cancer and partial colon resection according to location.Methods: Stool samples from forty-one subjects (10 in the control group, 10 in the right-sided colon cancer [RCC]group, 6 in the sigmoid colon cancer [SCC] group, 9 in the right colon resection [RCR] group and 6 in the sigmoid colon resection [SCR] group) were collected, and DNA was extracted. After terminal restriction fragment length polymorphism(T-RFLP) analysis, the samples were subjected to 16S rRNA gene amplicon sequencing, and the metabolic function of the microbiota was predicted using PICRUSt2.Results: T-RFLP analysis showed a reduced ratio of clostridial cluster XIVa in the SCC patients and clostridial cluster IXin the RCC patients, although these changes were not evident in the RCR or SCR patients. 16S rRNA gene amplicon sequencing demonstrated that the diversity of the gut microbiota in the RCC group was higher than that in the control group, and the diversity in the SCR group was significantly higher than that in the RCR group. Principal coordinate analysis (PCoA) revealed significant differences according to the group. Analyses of the microbiota revealed that Firmicutes was significantly dominant in the RCC group and that the SCC group had a higher abundance of Verrucomicrobia. At the genus level, linear discriminant analysis effect size (LEfSe) revealed several bacteria, such as Ruminococcaceae, Streptococcaceae, Clostridiaceae, Gemellaceae, and Desulfovibrio, in the RCC group and several oral microbiomes in the SCC group. Metabolic function prediction revealed that cholesterol transport- and metabolismrelated enzymes were specifically upregulated in the RCC group and that cobalamin metabolism-related enzymes were downregulated in the SCC group.本文/Department of Gastroenterology, Mie General Medical Center, Yokkaichi 510‑8561, Japan.13p
- 国立国会図書館永続的識別子
- info:ndljp/pid/13024272
- コレクション(共通)
- コレクション(障害者向け資料:レベル1)
- コレクション(個別)
- 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
- 収集根拠
- 博士論文(自動収集)
- 受理日(W3CDTF)
- 2023-10-11T15:41:06+09:00
- 記録形式(IMT)
- application/pdf
- オンライン閲覧公開範囲
- 国立国会図書館内限定公開
- デジタル化資料送信
- 図書館・個人送信対象外
- 遠隔複写可否(NDL)
- 可
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館デジタルコレクション