Overexpression of microRNA-345 Affects the Invasive Capacity of Pancreatic Ductal Adenocarcinoma Cell Lines by Suppressing MUC1 and TJP2 Expression

• 資料種別: 博士論文
• タイトル: Overexpression of microRNA-345 Affects the Invasive Capacity of Pancreatic Ductal Adenocarcinoma Cell Lines by Suppressing MUC1 and TJP2 Expression
• 著者・編者: Tatsumi, Shigenobu; Fujii, Tomomi; Sugimoto, Aya; Sekita-Hatakeyama, Yoko; Morita, Kohei; Uchiyama, Tomoko; Itami, Hiroe; Takeda, Maiko; Yamazaki, Masaharu; Sho, Masayuki; Ohbayashi, Chiho
• 著者標目: Tatsumi, Shigenobu; Fujii, Tomomi; Sugimoto, Aya; Sekita-Hatakeyama, Yoko; Morita, Kohei; Uchiyama, Tomoko; Itami, Hiroe; Takeda, Maiko; Yamazaki, Masaharu; Sho, Masayuki; Ohbayashi, Chiho
• 出版事項: MDPI
• 出版年月日等: 2022-05-25
• 出版年（W3CDTF）: 2022-05-25
• 並列タイトル等: microRNA-345の過剰発現は、MUC1およびTJP2の発現を抑制することにより、膵管腺癌細胞株の浸潤能に影響を及ぼす
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2023-03-15
• 授与年月日（W3CDTF）: 2023-03-15
• 報告番号: 甲第866号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: PDAC; MUC1; TJP2; miR-345; cell proliferation; invasion
• 対象利用者: 一般
• 一般注記: type:Thesis; The majority of pancreatic carcinomas are pancreatic ductal adenocarcinomas (PDAC), andthe presence of non-invasive pancreatic intraepithelial neoplasia or intraductal papillary mucinousneoplasm, as an associated lesion, is considered important. These microscopic hyperplastic or grosslypapillomatous lesions exhibit varying degrees of morphological atypia and may develop into invasivecarcinomas. In this study, we investigated whether mucin-1 (MUC1) is involved in the progression ofpancreatic carcinoma and examined the mechanisms by which microRNAs regulate MUC1 expressionin vitro. In PDAC cell lines, suppression of MUC1 expression reduced cell proliferation and invasion;PDAC cell lines transfected with an miR-345 precursor suppressed the expression of MUC1, andreduced cell proliferation and invasion. Tight junction protein 2 (TJP2), a putative target of miR-345,is regulated by MUC1. The suppression of TJP2 expression reduced cell proliferation by inducingapoptosis. These results suggest that MUC1 and TJP2, the putative target molecules of miR-345,are critical in maintaining the invasive potential of pancreatic carcinoma cells, and regulating theirexpression may prevent the progression of non-invasive pancreatic intraductal lesions to invasivecarcinomas. This study provides new insights for the development of novel molecular targetedtherapies for pancreatic carcinomas.; 博士（医学）・甲第866号・令和5年3月15日; © 2022 by the authors. Licensee MDPI, Basel, Switzerland.This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).; identifier:Applied Sciences Vol.12 No.11 Article No.5351 (2022 May); identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4098; identifier:Applied Sciences, 12(11): Article No.5351
• 国立国会図書館永続的識別子: info:ndljp/pid/13120210; https://dl.ndl.go.jp/pid/13120210
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2023-12-05T21:41:07+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10564/4098
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp