並列タイトル等脳皮質静脈梗塞ラットモデルを用いた脳静脈虚血による神経細胞および神経前駆細胞の発現と分布
一般注記type:Thesis
Neurogenesis in the subventricular zone (SVZ), subgranular zone (SGZ), and cerebral cortex is now a familiarevent to confirm by cerebral arterial ischemia in rat models. However, it remains unclear whether cerebralvenous ischemia (CVI) alone causes neurogenesis, and where that neurogenesis occurs. After creating CVI ratmodels via a two-vein occlusion (2-VO) method, neurogenesis was immunohistochemically evaluated by doublelabeling5-bromo-2′ -deoxyuridine (BrdU)-positive cells with neuronal nuclei (NeuN) or doublecortin (DCX)antibody. Fifty Wistar rats were divided into two major groups (BrdU-NeuN and BrdU-DCX) and then separatedinto two subgroups (2-VO or sham). The total number of double-positive cells expressed inside a predefinedregion of interest (ROI) covering the ischemic area was compared between the two subgroups. Then, we dividedthe ROI into six sections to evaluate and compare the distribution of double-positive cells generated in eachsection between the two subgroups. The 2-VO subgroup presented more double-positive cells than the shamgroup in both BrdU-NeuN and BrdU-DCX groups, while the BrdU-DCX+2-VO group showed a characteristicdistribution of double-positive cells in ROI 2 and ROI 3, suggesting areas of the ischemic core and penumbra,with a significant difference compared to the BrdU-DCX+sham group. This study demonstrates that CVI has thepotential to induce endogenous neurogenesis, with significant numbers of both newly generated neurons andprecursors observed in the ischemic area. The distribution of these cells suggests that the cortex could be themain origin of neurogenesis after cortical CVI.
博士（医学）・甲第872号・令和5年3月15日
© 2022 The Authors. Published by Elsevier Ltd on behalf of International Brain Research Organization. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
identifier:IBRO neuroscience reports Vol.14 p.50-56 (2022 Dec)
identifier:26672421
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4104
identifier:IBRO neuroscience reports, 14: 50-56
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