Mass spectrometric and immunological evaluation of AGEs during incubation of ribose with gelatin

• 資料種別: 記事
• タイトル: Mass spectrometric and immunological evaluation of AGEs during incubation of ribose with gelatin
• 著者・編者: Nana Katsuta; Mime Nagai; Ryoji Nagai
• 出版事項: 日本 : 糖化ストレス研究会
• 出版年月日等: 2023-03-31
• 出版年（W3CDTF）: 2023-03-31
• タイトル（掲載誌）: Glycative stress research
• 巻号年月日等（掲載誌）: 10(1)
• 掲載巻: 10(1)
• ISSN（掲載誌）: 2188-3610
• ISSN-L（掲載誌）: 2188-3610
• 本文の言語コード: eng
• DOI: 10.24659/gsr.10.1_16; https://doi.org/10.24659/gsr.10.1_16
• 国立国会図書館永続的識別子: info:ndljp/pid/13120795; https://dl.ndl.go.jp/pid/13120795
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• 収集根拠: オンライン資料収集制度
• 受理日（W3CDTF）: 2023-12-08T11:19:22+09:00
• 保存日（W3CDTF）: 2023-05-20
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: https://www.toukastress.jp/webj/
• 掲載誌（国立国会図書館永続的識別子）: info:ndljp/pid/13120792; https://dl.ndl.go.jp/pid/13120792
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• コレクション（個別）: 国立国会図書館デジタルコレクション > 電子書籍・電子雑誌 > その他; https://dl.ndl.go.jp/collections/B00035
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 所蔵機関: 国立国会図書館
• 請求記号: Z63-D541
• 関連情報（URI）: https://dl.ndl.go.jp/info:ndljp/pid/13120795
• 関連情報（国立国会図書館永続的識別子）: info:ndljp/pid/13120795; https://dl.ndl.go.jp/info:ndljp/pid/13120795
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館雑誌記事索引; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 033430112; http://id.ndl.go.jp/bib/033430112
• 整理区分コード: 632

• 要約等: Ribose produces advanced glycation end-products (AGEs) more rapidly than glucose. It has also been reported that <i>N^ε</i>-(carboxymethyl)arginine (CMA) is formed in collagen, which is the most abundant protein in organisms. In this study, AGEs produced by the reaction of ribose with gelatin, a soluble collagen, were evaluated by immunochemical methods using anti-AGEs antibodies, mass spectrometry, and fluorescence intensity measurements. Measurement by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that <i>N^ε</i>-(carboxymethyl)lysine (CML), <i>N^δ</i>-(5-hydro-5-methyl-4-imidazolone-2-yl)-ornithine (MG-H1) and CMA were significantly increased in the ribose-gelatin sample compared to the gelatin alone sample after a 7-day incubation at 37°C. The fluorescence intensity at excitation and emission of 370 nm and 440 nm, respectively, was also significantly increased, whereas the AGE structure detected was unknown. In addition, the pericarp hot water extract of<i>Trapa bispinosa</i> (TBE) and/or lutein were added to this reaction system to evaluate the inhibitory effect of AGE formation. As a result, inhibition of CML, MG-H1, and CMA formation was observed at ≥ 5 μg/mL of TBE in ELISA and LC-MS/MS. However, no similar inhibitory effect was detected in fluorescence measurements, suggesting that TBE does not inhibit the formation of fluorescent AGEs. Since the inhibition of AGE formation by lutein was not confirmed, it may be a difficult compound to evaluate by in vitro studies. This study showed that ELISA and LC-MS/MS measurement can detect three types of AGEs formation in the ribose-gelatin system and that they are useful for screening compounds, e.g., TBE, that inhibit AGE formation.
• DOI: 10.24659/gsr.10.1_16
• オンライン閲覧公開範囲: インターネット公開
• 連携機関・データベース: 科学技術振興機構 : J-STAGE; http://www.jstage.jst.go.jp

• 要約等: Ribose produces advanced glycation end-products (AGEs) more rapidly than glucose. It has also been reported that <i>N^ε</i>-(carboxymethyl)arginine (CMA) is formed in collagen, which is the most abundant protein in organisms. In this study, AGEs produced by the reaction of ribose with gelatin, a soluble collagen, were evaluated by immunochemical methods using anti-AGEs antibodies, mass spectrometry, and fluorescence intensity measurements. Measurement by enzyme-linked immunosorbent assay (ELISA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that <i>N^ε</i>-(carboxymethyl)lysine (CML), <i>N^δ</i>-(5-hydro-5-methyl-4-imidazolone-2-yl)-ornithine (MG-H1) and CMA were significantly increased in the ribose-gelatin sample compared to the gelatin alone sample after a 7-day incubation at 37°C. The fluorescence intensity at excitation and emission of 370 nm and 440 nm, respectively, was also significantly increased, whereas the AGE structure detected was unknown. In addition, the pericarp hot water extract of<i>Trapa bispinosa</i> (TBE) and/or lutein were added to this reaction system to evaluate the inhibitory effect of AGE formation. As a result, inhibition of CML, MG-H1, and CMA formation was observed at ≥ 5 μg/mL of TBE in ELISA and LC-MS/MS. However, no similar inhibitory effect was detected in fluorescence measurements, suggesting that TBE does not inhibit the formation of fluorescent AGEs. Since the inhibition of AGE formation by lutein was not confirmed, it may be a difficult compound to evaluate by in vitro studies. This study showed that ELISA and LC-MS/MS measurement can detect three types of AGEs formation in the ribose-gelatin system and that they are useful for screening compounds, e.g., TBE, that inhibit AGE formation.
• DOI: 10.24659/gsr.10.1_16; https://doi.org/10.24659/gsr.10.1_16
• 関連情報（URI）: http://id.ndl.go.jp/bib/033430112; https://ndlsearch.ndl.go.jp/books/R000000004-I033430112; https://dl.ndl.go.jp/pid/13120795; http://id.ndl.go.jp/digimeta/13120795; https://search.jamas.or.jp/link/ui/2024180406
• 連携機関・データベース: 国立情報学研究所 : CiNii Research; https://cir.nii.ac.jp/
• 提供元機関・データベース: Japan Link Center; https://japanlinkcenter.org/top; 雑誌記事索引データベース; https://ndlsearch.ndl.go.jp
• 書誌ID（NDLBibID）: 033430112; http://id.ndl.go.jp/bib/033430112; 13120795; http://id.ndl.go.jp/bib/13120795