Alteration in melanin content in retinal pigment epithelial cells upon hydroquinone exposure 23

• 資料種別: 博士論文
• タイトル: Alteration in melanin content in retinal pigment epithelial cells upon hydroquinone exposure
• 巻次・部編番号: 24 23
• 著者・編者: Nishiyama, Takeyuki; Tsujinaka, Hiroki; Ueda, Tetsuo; Ogata, Nahoko
• 著者標目: Nishiyama, Takeyuki; Tsujinaka, Hiroki; Ueda, Tetsuo; Ogata, Nahoko
• 出版事項: MDPI
• 出版年月日等: 2023-11; 24 23
• 出版年（W3CDTF）: 2023-11
• 並列タイトル等: ハイドロキノン曝露による網膜色素上皮細胞のメラニン含量の変化
• タイトル（掲載誌）: International Journal of Molecular Sciences
• 掲載ページ: 16801-
• ISSN（掲載誌）: 16616596
• 授与機関名: 奈良県立医科大学
• 授与年月日: 2024-03-14
• 授与年月日（W3CDTF）: 2024-03-14
• 報告番号: 甲第920号
• 学位: 博士（医学）
• 本文の言語コード: eng
• 件名標目: neovascular age-related macular degeneration; melanin production; hydroquinone; vascular endothelial growth factor; blue light
• 対象利用者: 一般
• 一般注記: type:Thesis; Abnormal pigmentation or depigmentation of the retinal pigment epithelium (RPE) is a precursor to neovascular age-related macular degeneration (nAMD). In this study, we evaluated the effects of hydroquinone (HQ), the most potent reductant in cigarette smoke, on the melanin production in RPE cells. Induced pluripotent stem cell (iPS)-derived RPE and adult retinal pigment epithelial (ARPE-19) cells were cultured with HQ. Real-time reverse transcription polymerase chain reaction revealed that the expression of melanin-related genes decreased due to the addition of HQ for 1 day. Enzyme-linked immunosorbent immunoassay showed that the concentration of melanin significantly decreased due to the addition of HQ for 24 h. A suspension of RPE cells with HQ for 24 h was prepared, and the absorbance was measured. The absorbance decreased particularly under blue light, suggesting that blue light may reach the choroid and cause choroidal inflammation. Additionally, melanin levels significantly decreased due to the addition of HQ for 1 week. After blue light irradiation on the RPE with HQ for 1 week, the vascular endothelial growth factor in the medium was significantly higher in the HQ group than in the control group. HQ-induced changes in melanin production may be responsible for the uneven pigmentation of the RPE, and these changes may cause nAMD.; 権利情報：© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).; identifier:International Journal of Molecular Sciences. 2023 Nov, vol.24, no.23, article no.16801; identifier:1661-6596; identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/4371; identifier:International Journal of Molecular Sciences, 24(23): 16801
• 国立国会図書館永続的識別子: info:ndljp/pid/13722145; https://dl.ndl.go.jp/pid/13722145
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2024-07-05T22:06:56+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 関連情報（URI）: https://doi.org/10.3390/ijms242316801
• 掲載誌（URI）: http://hdl.handle.net/10564/4371
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp