並列タイトル等Silver nanoparticles reduce ACE2 expression via changing mitochondrial function in human fibroblast-like lung cell and periodontal ligament fibroblast cells
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博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨
Silver nanoparticles (AgNPs) have demonstrated antibacterial properties and are widely recognized as one of the most prominent types of nanoparticles. Recent studies have highlighted their effectiveness against coronaviruses. However, the detailed molecular mechanisms underlying the action of AgNPs on viruses and their impacts on the human body remain to be fully elucidated. Thus, we attempt to delineate the preventive effects of AgNPs against SARS-CoV-2 infection. Our findings indicate that treatment with AgNPs reduces ACE2 expression, a key receptor for SARS-CoV-2 particularly in normal oral and lung cells. Additionally, we observed a decrease in the binding affinity of the spike protein to the cell after the AgNP treatment. Through western blot analysis, we identified the involvement of the AKT and/or mTOR signaling pathway in this process. Because AKT and mTOR signaling affects mitochondrial function, we investigated the effects of the AgNP treatment on mitochondria. As a result, we observed the localization of AgNPs within mitochondria. Further, an increase in mitochondrial Fe2+ and reactive oxygen species levels was observed, ultimately resulting in mitochondrial dysfunction. Our results show the efficacy of the AgNP treatment in preventing coronavirus infections.
Shosei Takahashi, Kazuo Tomita, Kento Igarashi, Yoshikazu Kuwahara, Junichi Kitanaka, Nobue Kitanaka, Koh-ichi Tanaka, Akihiro Kurimasa, Yoshiaki Kamikawa & Tomoaki SatoSilver Nanoparticles Reduce ACE2 Expression via Changing Mitochondrial Function in Human Fibroblast-Like Lung Cell and Periodontal Ligament Fibroblast CellsBioNanoSci. 15, 39 (2025)https://doi.org/10.1007/s12668-024-01630-7
The version of record of this article, first published in BioNanoScience, is available online at Publisher’s website: https://doi.org/10.1007/s12668-024-01630-7
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