胃癌患者の血中の pre-microRNA-488 の臨床的意義の解明

• 資料種別: 博士論文
• タイトル: 胃癌患者の血中の pre-microRNA-488 の臨床的意義の解明
• 著者・編者: 鶴田, 祐介
• 著者標目: 鶴田, 祐介
• 出版事項: International Institute of Anticancer Research
• 出版年月日等: 2025-03-19
• 出版年（W3CDTF）: 2025-03-19
• 並列タイトル等: Circulating pre-microRNA-488 in blood is a potential prognostic biomarker in gastric cancer
• 授与機関名: 鹿児島大学
• 授与年月日: 2025-03-19
• 授与年月日（W3CDTF）: 2025-03-19
• 報告番号: 甲総研第767号
• 学位: 博士(医学)
• 本文の言語コード: eng
• 件名標目: Pre-miR-488; miR-488-5p; gastric cancer; biomarker; liquid biopsy
• 対象利用者: 一般
• 一般注記: 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Background/Aim: MicroRNAs (miRNAs) have been highlighted as non-invasive clinical biomarkers in liquid biopsy. This study aimed to investigate the clinical significance of circulating tumor suppressors, precursor-miR-488 (pre-miR-488) and miR-488-5p, in the blood of patients with gastric cancer (GC). Materials and Methods: The expression levels of pre-miR-488 and miR-488-5p in tumor tissues and blood were measured using RT-qPCR, and the clinicopathological and prognostic significance of their expression was assessed in patients with GC. Next, pathway analysis of miR-488-5p expression in GC tissues was performed by gene set enrichment analysis (GSEA) using the TCGA dataset. Finally, pre-miR-488 in exosomes from the plasma of GC patients was analyzed using RT-qPCR. Results: Both pre-miR-488 and miR-488-5p were down-regulated in tumor tissues, whereas their expression in the blood was significantly higher in patients with GC than in healthy controls. Low expression of pre-miR-488 or miR-488-5p in the blood was associated with poor prognosis in patients with GC. Furthermore, low pre-miR-488 expression in the blood was an independent poor prognostic factor for overall survival. miR-488-5p expression tended to be negatively correlated with the expression of gene sets involved in epithelial-mesenchymal transition and hypoxia. pre-miR-488 was detected in exosomes isolated from the plasma of patients with GC. Conclusion: Circulating pre-miR-488 and miR-488-5p expression in the blood may serve as novel prognostic biomarkers for patients with GC, predicting clinical outcomes and guiding therapeutic strategies.; YUSUKE TSURUDA, TAKAAKI MASUDA, YOSHIKI HIRAKI, MIWA KITAGAWA, DAI SHIMIZU, QINGJIANG HU, SHUHEI ITO, HAJIME OTSU, YOSUKE KURODA, HIDETOSHI EGUCHI, TAKAO OHTSUKA, SHOJI NATSUGOE and KOSHI MIMORICirculating Pre-microRNA-488 in Blood Is a Potential Prognostic Biomarker in Gastric CancerAnticancer Research January 2025, 45 (1) 123-133https://doi.org/10.21873/anticanres.17399
• 国立国会図書館永続的識別子: info:ndljp/pid/14551117; https://dl.ndl.go.jp/pid/14551117
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2025-11-07T22:06:05+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://hdl.handle.net/10232/0002000847
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp