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博士論文
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DOI[info:doi/10.1186/1477-5956-11-13]のデータに遷移します
Profiling and annotation of human kidney glomerulus proteome
- 国立国会図書館永続的識別子
- info:ndljp/pid/8948577
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- Background: The comprehensive analysis of human kidney glomerulus we previously performed using highly purified glomeruli, provided a dataset of 6,686...
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デジタル
- 資料種別
- 博士論文
- 著者・編者
- Cui, Zhenyu
- 出版事項
- 出版年月日等
- 2013-09-20
- 出版年(W3CDTF)
- 2013-09-20
- 並列タイトル等
- Profiling and annotation of human kidney glomerulus proteomeヒト腎糸球体プロテオームの網羅的解析とその特徴について
- 授与機関名
- 新潟大学
- 授与年月日
- 2013-09-20
- 授与年月日(W3CDTF)
- 2013-09-20
- 報告番号
- 甲第3810号
- 学位
- 博士(医学)
- 博論授与番号
- 甲第3810号
- 本文の言語コード
- eng
- 件名標目
- 一般注記
- Background: The comprehensive analysis of human kidney glomerulus we previously performed using highly purified glomeruli, provided a dataset of 6,686 unique proteins representing 2,966 distinct genes. This dataset, however, contained considerable redundancy resulting from identification criteria under which all the proteins matched with the same set of peptides and its subset were reported as identified proteins. In this study we reanalyzed the raw data using the Mascot search engine and highly stringent criteria in order to select proteins with the highest scores matching peptides with scores exceeding the “Identity Threshold” and one or more unique peptides. This enabled us to exclude proteins with lower scores which only matched the same set of peptides or its subset. This approach provided a high-confidence, non-redundant dataset of identified proteins for extensive profiling, annotation, and comparison with other proteome datasets that can provide biologically relevant knowledge of glomerulus proteome. Results: Protein identification using the Mascot search engine under highly stringent, computational strategy generated a non-redundant dataset of 1,817 proteins representing 1,478 genes. These proteins were represented by 2-D protein array specifying observed molecular weight and isoelectric point range of identified proteins to demonstrate differences in the observed and calculated physicochemical properties. Characteristics of glomerulus proteome could be illustrated by GO analysis and protein classification. The depth of proteomic analysis was well documented via comparison of the dynamic range of identified proteins with other proteomic analyses of human glomerulus, as well as a high coverage of biologically important pathways. Comparison of glomerulus proteome with human plasma and urine proteomes, provided by comprehensive analysis, suggested the extent and characteristics of proteins contaminated from plasma and excreted into urine, respectively. Among the latter proteins, several were demonstrated to be highly or specifically localized in the glomerulus by cross-reference analysis with the Human Protein Atlas database, and could be biomarker candidates for glomerular injury. Furthermore, comparison of ortholog proteins identified in human and mouse glomeruli suggest some biologically significant differences in glomerulus proteomes between the two species. Conclusions: A high-confidence, non-redundant dataset of proteins created by comprehensive proteomic analysis could provide a more extensive understanding of human glomerulus proteome and could be useful as a resource for the discovery of biomarkers and disease-relevant proteins.学位の種類: 博士(医学). 報告番号: 甲第3810号. 学位記番号: 新大院博(医)甲第562号. 学位授与年月日: 平成25年9月20日新大院博(医)甲第562号元資料の権利情報 : © 2013 Cui et al.; licensee BioMed Central Ltd.
- DOI
- info:doi/10.1186/1477-5956-11-13
- 国立国会図書館永続的識別子
- info:ndljp/pid/8948577
- コレクション(共通)
- コレクション(障害者向け資料:レベル1)
- コレクション(個別)
- 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
- 収集根拠
- 博士論文(自動収集)
- 公開開始日(W3CDTF)
- 2015-03-03
- 受理日(W3CDTF)
- 2015-02-03T05:25:05+09:00
- 作成日(W3CDTF)
- 2019-08-05
- 記録形式(IMT)
- application/pdf
- オンライン閲覧公開範囲
- 国立国会図書館内限定公開
- デジタル化資料送信
- 図書館・個人送信対象外
- 遠隔複写可否(NDL)
- 可
- 連携機関・データベース
- 国立国会図書館 : 国立国会図書館デジタルコレクション