Identification of novel transmembrane proteins in scirrhous type gastric cancer by Escherichia coli ampicillin secretion trap (CAST) method : TM9SF3 participates in tumor invasion and serves as a prognostic factor

• 資料種別: 博士論文
• タイトル: Identification of novel transmembrane proteins in scirrhous type gastric cancer by Escherichia coli ampicillin secretion trap (CAST) method : TM9SF3 participates in tumor invasion and serves as a prognostic factor
• 著者・編者: Oo, Htoo Zarni
• 著者標目: Oo, Htoo Zarni
• 出版年月日等: 2014-03-23
• 出版年（W3CDTF）: 2014-03-23
• 並列タイトル等: CAST法を用いた新規スキルス胃癌特異的膜蛋白質の同定 : TM9SF3は癌の浸潤に関与し、 予後因子となる
• 授与機関名: 広島大学
• 授与年月日: 2014-03-23
• 授与年月日（W3CDTF）: 2014-03-23
• 報告番号: 甲第6397号
• 学位: 博士(医学); Philosophy in Medical Science
• 博論授与番号: 甲第6397号
• 本文の言語コード: eng
• 件名標目: CAST; Gastric cancer; TM9SF3; Prognosis; Transmembrane 9 superfamily member 3
• NDC: 490
• 対象利用者: 一般
• 一般注記: Objective: Scirrhous type gastric cancer is highly aggressive and has a worse prognosis because of its rapid cancer cell infiltration, accompanied by extensive stromal fibrosis. The aim of this study is to identify genes that encode transmembrane proteins frequently expressed in scirrhous type gastric cancer. Methods: We generated Escherichia coli ampicillin secretion trap (CAST) libraries from 2 human scirrhous type gastric cancer tissues and compared with a normal stomach CAST library. By sequencing 2,880 colonies from scirrhous CAST libraries, we identified a list of candidate genes. Results: We focused on TM9SF3 gene because it has the highest clone count and immunohistochemical analysis demonstrated that 46 (50%) of 91 gastric cancer cases were positive for TM9SF3 and it was observed frequently in scirrhous type gastric cancer. TM9SF3 expression showed a significant correlation with the depth of invasion, tumor stage and undifferentiated type of gastric cancer. There was a strong correlation between TM9SF3 expression and poor survival prognosis of patients, validated in two separate cohorts, by immunostaining or qRT‐PCR. Transient knockdown of the TM9SF3 gene by siRNA showed decreased tumor cell invasive capacity. Conclusion: Our results indicate that TM9SF3 might be a potential diagnostic and therapeutic target for scirrhous type gastric cancer.; Htoo Zarni Oo, Kazuhiro Sentani, Naoy, Sakamoto, Katsuhiro Anami, Yutak, Naito, Takashi Oshima, Kazuyoshi Yanagihara, Naohide Oue and Wataru Yasui; Identification of novel transmembrane proteins in scirrhous-type gastric cancer by the Escherichia coli ampicillin secretion trap (CAST) method: TM9SF3 participates in tumor invasion and serves as a prognostic factor.; Pathobiology 2014, 81, 138-148 (DOI: 10.1159/000357821)
• 国立国会図書館永続的識別子: info:ndljp/pid/8952597; https://dl.ndl.go.jp/pid/8952597
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 公開開始日（W3CDTF）: 2015-03-03
• 受理日（W3CDTF）: 2015-02-03T05:25:05+09:00
• 作成日（W3CDTF）: 2014-11-21
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://ir.lib.hiroshima-u.ac.jp/00036310
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

目次

2018-12-03 再収集

2018-12-03 再収集