Pigment epithelium-derived factor inhibits the growth of human esophageal squamous cell carcinoma by suppressing neovascularization.

• 資料種別: 博士論文
• タイトル: Pigment epithelium-derived factor inhibits the growth of human esophageal squamous cell carcinoma by suppressing neovascularization.
• 著者・編者: 角谷, 昌俊
• 著者標目: 角谷, 昌俊
• 出版事項: Hokkaido University
• 出版年月日等: 2015-03-25
• 出版年（W3CDTF）: 2015-03-25
• 並列タイトル等: PEDFは血管新生を阻害することにより食道癌の増殖を抑制しうる
• 寄与者: 武冨, 紹信; 秋田, 弘俊; 平野, 聡
• 授与機関名: 北海道大学
• 授与年月日: 2015-03-25
• 授与年月日（W3CDTF）: 2015-03-25
• 報告番号: 甲第11646号
• 学位: 博士(医学)
• 博論授与番号: 甲第11646号
• 本文の言語コード: eng
• 件名標目: Pigment epithelium-derived factor; Esophageal squamous cell carcinoma; Angiogenesis; Vasculogenesis; Endothelial progenitor cell
• NDC: 490
• 対象利用者: 一般
• 一般注記: 配架番号：2128; Introduction: Neovascularization consists of angiogenesis and vasculogenesis during which bone marrow-derived endothelial progenitor cells (EPCs) are mobilized for blood vessel formation. Pigment epithelium-derived factor (PEDF) is known to be a potent inhibitor of angiogenesis in some solid carcinomas. However, the effects of PEDF on human esophageal squamous cell carcinoma (ESCC) and vasculogenesis are still unknown. The purpose of this research was to investigate the effect of PEDF on angiogenesis, tumor growth, and vasculogenesis in ESCC.Materials and Methods: The PEDF gene was transduced to the TE8 ESCC cell line not secreting endogenous PEDF and the HEC46 cell line originally secreting endogenous PEDF by lentivirus-based vectors expressing PEDF. In vitro endothelial cell proliferation and migration assays were performed using the supernatant derived from PEDFoverexpressing cells. In in vivo experiments, the effects of PEDF on chronological tumor growth, intratumoral microvessel density (MVD), tumor cell apoptosis, and the frequency of EPCs in peripheral blood and tumor tissues were examined in murine subcutaneous tumor models.Results: PEDF inhibited endothelial cell proliferation and migration in vitro and showed potent in vivo antitumor properties by inhibiting MVD in the human ESCC cell line that did not secrete endogenous PEDF. However, in the cell line secreting endogenous PEDF, additional PEDF gene transfer showed no inhibition of angiogenesis and no subsequent antitumor properties. With respect to vasculogenesis, PEDF was found to have potential to suppress vasculogenesis; the frequency of EPCs both in peripheral blood and tumor tissue was decreased in mice implanted with PEDFoverexpressing TE8 and HEC46 cells.Conclusion: PEDF may have potent antiangiogenic and antitumor effects in ESCC cells naturally not secretingendogenous PEDF and can be expected to be applied as gene therapy in the future.; (主査) 教授 武冨 紹信, 教授 秋田 弘俊, 教授 平野 聡; 医学研究科（癌医学専攻）
• DOI: 10.14943/doctoral.k11646; https://doi.org/10.14943/doctoral.k11646
• 国立国会図書館永続的識別子: info:ndljp/pid/9424902; https://dl.ndl.go.jp/pid/9424902
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2015-07-01T13:17:09+09:00
• 記録形式（IMT）: PDF
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://dx.doi.org/10.14943/doctoral.k11646; http://hdl.handle.net/2115/59123
• 参照（URI）: http://hdl.handle.net/2115/59122
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• 要約等: Introduction: Neovascularization consists of angiogenesis and vasculogenesis during which bone marrow-derived endothelial progenitor cells (EPCs) are ...