並列タイトル等Decreased urinary calbindin 1 levels in proteinuric rats and humans with distal nephron segment injuries
遠位尿細管傷害のラットとヒトにおける尿中calbindin1蛋白質排泄の減少について
一般注記"Background: Several proteins have been proposed as new urinary biomarkers of kidney injuries, but they are not always capable of identifying the kidney nephron segment that has been injured. Since calbindin 1 protein is exclusively localized in the kidney distal nephron segment, it is presumed that its expression is altered during distal nephron segment injuries, resulting in changes in its urinary excretion. Methods Calbindin 1 expression in normal rat kidneys was compared with that in the kidneys of rats that had suffered distal nephron segment injuries (unilateral ureteral obstruction [UUO] or anti-glomerular basement membrane glomerulonephritis [anti-GBM GN]) using immunohistochemical examinations and real-time polymerase chain reaction. The urinary calbindin 1 protein concentration of normal rats was also compared with that of anti-GBM GN rats and of cisplatin nephropathy rats using Western blotting. We also compared the kidney and urinary calbindin 1 protein concentrations of normal human subjects with those of proteinuric patients [immunoglobulin (Ig)A nephropathy; IgAN] with distal nephron segment injuries. Results: Calbindin 1 mRNA expression in the renal cortices and calbindin 1 protein expression in the kidney distal nephron segments were decreased in the UUO and anti-GBM GN rat kidneys. The urinary calbindin 1 protein levels of the anti-GBM GN rats were also markedly decreased, whereas those of the cisplatin nephropathy rats were slightly decreased. The human IgAN patients displayed decreased renal calbindin 1 protein expression in\
their dilated distal tubules, and some patients displayed decreased urinary calbindin 1 levels. Conclusion: Since it has been demonstrated that decreased urinary calbindin 1 levels are indicative of decreased calbindin 1 kidney expression due to distal nephron segment injuries, calbindin 1 might be a useful urinary biomarker for identifying distal nephron segment injuries."
学位の種類: 博士（医学）. 報告番号: 甲第3986号. 学位記番号: 新大院博（医）甲第632号. 学位授与年月日: 平成27年3月23日
Clinical and Experimental Nephrology. 2014, 18(3), 432-443.
新大院博（医）甲第632号
元資料の権利情報 : Copyright (C) 2013 Japanese Society of Nephrology
元資料の権利情報 : The final publication is available at www.springerlink.com
DOIinfo:doi/10.1007/s10157-013-0835-3
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