Histone Demethylase Jmjd3 Regulates Osteoblast Differentiation via Transcription Factors Runx2 and Osterix 47

• 資料種別: 博士論文
• タイトル: Histone Demethylase Jmjd3 Regulates Osteoblast Differentiation via Transcription Factors Runx2 and Osterix
• 巻次・部編番号: 288 47
• 著者・編者: Yang, Di; Okamura, Hirohiko; Nakashima, Yoshiki; Haneji, Tatsuji
• 著者標目: Yang, Di; Okamura, Hirohiko; Nakashima, Yoshiki; Haneji, Tatsuji
• 出版年月日等: 2013-10-08
• 出版年（W3CDTF）: 2013-10-08
• 並列タイトル等: ヒストンデメチラーゼJmjd3は転写因子Runx2とOsterixを介して骨芽細胞の分化を調節する; Regulation of osteoblast differentiation by Jmjd3
• タイトル（掲載誌）: The Journal of Biological Chemistry
• 授与機関名: 徳島大学
• 授与年月日: 2015-09-16
• 授与年月日（W3CDTF）: 2015-09-16
• 報告番号: 甲第2862号
• 学位: 博士（歯学）
• 博論授与番号: 甲第2862号
• 本文の言語コード: eng
• 件名標目: Jmjd3; H3K27me3; BMP-2; Osteoblast differentiation; Histone demethylation; Runx2; Osterix
• 対象利用者: 一般
• 一般注記: Post-translational modifications of histones including methylation play important roles in cell differentiation. Jumonji domain-containing 3 (Jmjd3) is a histone demethylase, which specifically catalyzes the removal of trimethylation of histone H3 at lysine 27 (H3K27me3). In this study, we examined the expression of Jmjd3 in osteoblasts and its roles in osteoblast differentiation. Jmjd3 expression in the nucleus was induced in response to the stimulation of osteoblast differentiation as well as treatment of bone morphogenetic protein-2 (BMP-2). Either treatment with Noggin, an inhibitor of BMP-2, or silencing of Smad1/5, suppressed Jmjd3 expression during osteoblast differentiation. Silencing of Jmjd3 expression suppressed osteoblast differentiation through the expression of bone-related genes including Runx2, Osterix, Osteopontin, Bone sialoprotein (BSP), and Osteocalcin (OCN). Silencing of Jmjd3 decreased the promoter activities of Runx2 and Osterix and increased the level of H3K27me3 on the promoter regions of Runx2 and Osterix. Introduction of the exogenous Runx2 and Osterix partly rescued osteoblast differentiation in the shJmjd3 cells. The present results indicate that Jmjd3 plays important roles in osteoblast differentiation and regulates the expressions of BSP and OCN via transcription factors Runx2 and Osterix.
• DOI: info:doi/10.1074/jbc.M113.497040; https://doi.org/info:doi/10.1074/jbc.M113.497040
• 国立国会図書館永続的識別子: info:ndljp/pid/9506712; https://dl.ndl.go.jp/pid/9506712
• コレクション（共通）: 障害者向け資料
• コレクション（障害者向け資料：レベル1）: テキストデータ
• コレクション（個別）: 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• 収集根拠: 博士論文（自動収集）
• 受理日（W3CDTF）: 2015-10-01T11:16:23+09:00
• 記録形式（IMT）: application/pdf
• オンライン閲覧公開範囲: 国立国会図書館内限定公開
• デジタル化資料送信: 図書館・個人送信対象外
• 遠隔複写可否（NDL）: 可
• 掲載誌（URI）: http://repo.lib.tokushima-u.ac.jp/109488
• 連携機関・データベース: 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp